SPECIFICATIONS
Excitation:
340 
380 nm 
Emission:
505 nm 
Kd:
4 mM
MW:
1127
Solubility:
DMSO
Synonyms: Sodium-binding benzofuran isophthalate – AM, sodium binding indicator
Product code: 1 mg: 3031B / 20x 50mg: 3031E
product description
PBFI is a UV-excitable, green ratiometric indicator for intracellular potassium (K⁺) measurements. Ex/Em: 340/505 nm can be used to measure K⁺-bound PBFI, and Ex/Em: 380/505 nm can be used to detect K⁺-free PBFI. It is 1.5X more selective for K⁺ over Na⁺, and works well in cytosolic conditions where [K⁺]/[Na⁺] is ~10. Ratiometry is optimal for imaging applications where quantification of intracellular K⁺ concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.
documentation
