PBFI AM

$365.00

Ratiometric potassium (K⁺) indicator, membrane permeable

 

Frequently bought together

SPECIFICATIONS

Excitation:

340  380 nm 

Emission:

505 nm 

Kd:

4 mM

MW:

1127

Solubility:

DMSO

Synonyms: Sodium-binding benzofuran isophthalate – AM, sodium binding indicator
Product code: 1mg: 2021B / 20x 50mg: 2021E

product description

PBFI is a UV-excitable, green ratiometric indicator for intracellular potassium (K⁺) measurements. Ex/Em: 340/505 nm can be used to measure K⁺-bound PBFI, and Ex/Em: 380/505 nm can be used to detect K⁺-free PBFI. It is 1.5X more selective for K⁺ over Na⁺, and works well in cytosolic conditions where [K⁺]/[Na⁺] is ~10. Ratiometry is optimal for imaging applications where quantification of intracellular K⁺ concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.

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