340 380 nm
Synonyms: Sodium-binding benzofuran isophthalate – AM, sodium binding indicator
Product code: 1 mg: 3031B / 20x 50mg: 3031E
PBFI is a UV-excitable, green ratiometric indicator for intracellular potassium (K⁺) measurements. Ex/Em: 340/505 nm can be used to measure K⁺-bound PBFI, and Ex/Em: 380/505 nm can be used to detect K⁺-free PBFI. It is 1.5X more selective for K⁺ over Na⁺, and works well in cytosolic conditions where [K⁺]/[Na⁺] is ~10. Ratiometry is optimal for imaging applications where quantification of intracellular K⁺ concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.
Bezine M, Debbabi M, Nury T, Ben-Khalifa R, Samadi M, Cherkaoui-Malki M, Vejux A, Raas Q, de Sèze J, Moreau T, El-Ayeb M, Lizard G. Evidence of K+ homeostasis disruption in cellular dysfunction triggered by 7-ketocholesterol, 24S-hydroxycholesterol, and tetracosanoic acid (C24:0) in 158N murine oligodendrocytes. Chem Phys Lipids. 2017 Oct;207(Pt B):135-150. doi: 10.1016/j.chemphyslip.2017.03.006