Solute carrier transporters (SLC) facilitate uptake and efflux of a wide range of ions, metabolites and nutrients critical for maintaining cellular homeostasis, by coupling transport mechanisms to ion gradients.
These SLC transporters include SLC1, SLC5, SLC6, SLC8, SLC9, SLC20, SLC23, and SLC24 to name a few examples. They are known to shuttle nutrients, amino acids, neurotransmitters, ions and more across cellular membranes, and are being therapeutically targeted for conditions ranging from psychiatric disorders to kidney function.
ION Biosciences’ Sodium-dependent SLC Transporter Assay utilizes ION Natrium Green 2 (ING-2 AM), a sodium-sensitive fluorescent indicator, to detect solute carrier (SLC) transporter mediated sodium influx. This assay kit provides a highly-sensitive, robust, and high-throughput method to measure sodium-dependent SLC transporter activity.
(EX 525 nm, EM 545 nm) ION’s Sodium-dependent SLC Transporter Assay detects SLC transporter activity of SLCs that cotransport sodium, such as the SLC6 transporter family. This assay is ideal for hit finding and lead optimization applications in drug discovery, and can be used to discover substrates, potentiators, and inhibitors of sodium-dependent SLC transporters
ION Biosciences’ Brilliant Lithium Assay is a next-generation, fluorescence-based approach for high-throughput screening of NaV1.X channel modulators. The assay uses ION Lithium Green 1 AM (ILG-1 AM), a membrane-permeable, lithium-sensitive fluorescent dye that enables functional voltage gated sodium channel measurements using lithium as a surrogate ion. Lithium readily permeates open NaV1.X channels while bypassing many endogenous sodium-handling mechanisms, resulting in a robust and selective readout of channel activity. Upon depolarization or pharmacological activation of NaV1.X channels, lithium accumulates intracellularly producing a rapid, quantifiable fluorescence increase that reflects real time channel activity
The SLC12 family facilitates the movement of ions across membranes for cell volume maintenance, salt transport, and neurotransmission regulation. SLC12 transporters are electroneutral, and include NKCC1/2 and KCC1/2/3/4.
Thallium flux assays have been developed to screen for potentiators and inhibitors of some members of this family. We manufacture a collection of thallium flux assays that could be used to identify modulators of SLC12 transporters.
(EX 490 nm, EM 515 nm) High-throughput, no wash thallium flux assay. An optimal solution for measuring the activity of potassium channels and transporters, as well as sodium channels, non-selective cation channels, and Gi/o GPCRs.
(EX 530 nm, EM 550 nm) Brilliant Thallium Gold includes Thallos Gold – ION’s gold fluorescent thallium (Tl+) indicator. Red-shifted excitation and emission enable multiplexing with GFP-expressing cells or other green fluorescent indicators. Useful for fluorescence-based potassium channel assays and for studying a wide variety of monovalent cation channels (sodium channels), transporters, and GPCRs.
Proton-coupled transporters include the SLC2, SLC11, and SLC15 subfamilies, and potentially more. They are known to shuttle sugar alcohols, peptides, and metal ions across cellular membranes while cotransporting protons. These assays measure changes in intracellular pH using a membrane permeable, ratiometric, fluorescent indicator like BCECF AM, which has a pH-dependent ratio of emission intensity when the dye is excited at 490 nm, relative to 440 nm. The ratio of these emission intensities is directly proportional to the intracellular pH and can be used to quantify co-transport of other substrates in proton-coupled SLC transporters.
ION Biosciences’ Brilliant Intracellular pH Assay is a high-throughput, no-wash, fluorescence-based solution optimized to monitor activation of plasma membrane proton-coupled channels and transporters in response to pharmacological compounds. These include the voltage-gated proton channel (HV1), Na⁺/H⁺ exchangers (SLC9), divalent metal transporters (SLC11), peptide transporters (SLC15), monocarboxylate transporters (SLC16), proton-coupled amino acid transporters (SLC36), proton-coupled sugar transporters (SLC45), and the proton-coupled folate transporter (SLC46).
Kit contains enough reagents to conduct 10 plate-based assays.
