$182.00
Gq -coupled G protein-coupled receptors (GPCRs) are a subset of GPCRs that couple with the Gαq protein subunit.
Gα16 is a promiscuous Gαq protein subunit that can couple to a wide variety of receptors including Gαi/o and Gα12/13 GPCRs. The downstream signaling cascade of GPCR ligand binding includes activation of phospholipase C-β, an increase in IP3, and a release of calcium from intracellular stores like other Gq-coupled GPCRs. This chimeric G protein can be over-expressed in HEK293 and other mammalian cell lines to enable calcium signaling as a functional readout of GPCR activity, and is commonly used to uncover native ligands for orphan GPCRs.
Our Flex kit provides all necessary reagents in a format to maximize flexibility for your assay design and customization challenges. Brilliant Calcium Flex is a (EX 490 nm, EM 515 nm) high-throughput, no wash calcium mobilization assay. The most flexible solution for measuring the activity of GPCRs coupled to intracellular Ca²⁺ release.
(EX 525 nm, EM 550 nm, Kd 400nM) Brilliant Calcium Gold is a calcium flux assay for multi-well plate-based, high throughput measurements of calcium mobilization mediated through a wide variety of plasma membrane and intracellular calcium channels. The kit includes our Fluo-Gold dye, which is red-shifted for compatibility with GFP and other green fluorescent indicators.
Ratiometric Calcium Essentials kit provides the necessary reagents for conducting no wash, ratiometric calcium flux assays compatible with plate reader and fluorescence microscopy applications. Individual components are provided to give the user the flexibility needed to customize your assay.
(EX 340/380 nm, EM 505 nm, Kd 145nM) High-throughput, no wash ratiometric calcium (Ca²⁺) assay. No wash, compatible with plate reader and fluorescence microscopy applications.
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Brilliant Calcium High-throughput, no wash calcium (Ca²⁺) assay. An optimal solution for measuring intracellular Ca²⁺ dynamics, including effectors such as GPCRs and protein transporters. |
$182.00 – $3,069.00 | |||||
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Brilliant Calcium Gold ION Biosciences Brilliant Calcium Gold Assay is a total assay solution for multi-well plate-based, high-throughput measurements of Ca²⁺ mobilization mediated through a wide-variety of plasma membrane and intracellular calcium channels. The kit includes our Fluo-Gold dye (Ex/Em: 525/550 nm), which is red-shifted for compatibility with GFP and other green fluorescent indicators. |
$182.00 – $399.00 | |||||
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ION Essentials Ratiometric Calcium Combines Fura-2 AM with the necessary reagents for conducting no wash, ratiometric calcium flux assays compatible with plate reader and fluorescence microscopy applications. Individual components are provided to give you the flexibility needed to customize your assay. |
$170.00 – $374.00 |
