ION Essentials Ratiometric Calcium

Name: ION Essentials Ratiometric Calcium | Ca²⁺ Flux Assay | ION Biosciences
SKU: 6000
Availability: InStock

Price range: $178.00 through $392.00

Combines Fura-2 AM with the necessary reagents for conducting no wash, ratiometric calcium flux assays compatible with plate reader and fluorescence microscopy applications. Individual components are provided to give you the flexibility needed to customize your assay.

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Variation	SKU	Price	Quantity	Action
Size: 2 plates	6000-2	$178.00	ION Essentials Ratiometric Calcium quantity	Add to cart
Size: 10 plates	6000-10	$392.00	ION Essentials Ratiometric Calcium quantity	Add to cart

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Ratiometric calcium assay kit, using Fura-2 AM, packaging

SKU: 6000 Categories: Calcium, GPCR Assays, Gαq GPCR assays, Ion Channels and Transporter Assays Synonyms: calcium, essentials, ratiometric, Ratiometric Calcium Essential, Ratiometric Calcium Essentials Kit, variable

Specifications

Excitation: 340 nm, 380 nm

Emission: 505 nm

Kd: 145 nM

MW: 1002 g/mol

Solubility: DMSO

Purity: >95%

Filter Set: GFP/FITC, PI/Texas Red

Storage: -20°C

Size: 10 plates, 2 plates

Product Description

Ratiometric Calcium Essentials kit provides the necessary reagents for conducting no wash, ratiometric calcium flux assays compatible with plate reader and fluorescence microscopy applications. Individual components are provided to give the user the flexibility needed to customize your assay.

Ratiometric calcium assay data slide showing ratiometric images of Fura-2 before and after the addition of ionomycin.

Calcium flux assay data acquired using ION's ratiometric calcium kit. Dose response to ionomycin, compared to FLIPR kit. GPCR assay, muscarinic receptor, showing a dose response to carbachol.

Fura-2 is the most popular UV-excitable, ratiometric green indicator for intracellular calcium (Ca²⁺) measurements. Ex/Em: 340/505 nm can be used to measure Ca²⁺-bound Fura-2, and Ex/Em: 380/505 nm can be used to detect Ca²⁺-free Fura-2.

Ratiometry is not only optimal for imaging applications where quantification of intracellular Ca²⁺ concentrations is desired, it also reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology

Documentation

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