$505.00
ION Biosciences’ Brilliant Intracellular pH Assay is a high-throughput, no-wash, fluorescence-based solution optimized to monitor activation of plasma membrane proton-coupled channels and transporters in response to pharmacological compounds. These include the voltage-gated proton channel (HV1), Na⁺/H⁺ exchangers (SLC9), divalent metal transporters (SLC11), peptide transporters (SLC15), monocarboxylate transporters (SLC16), proton-coupled amino acid transporters (SLC36), proton-coupled sugar transporters (SLC45), and the proton-coupled folate transporter (SLC46).
Kit contains enough reagents to conduct 10 plate-based assays.
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Emission: 535 nm
Solubility: DMSO
Filter Set: BCECF (ratiometric), FITC (non-ratiometric)
Storage: Various conditions
Size: 10 plates
Intracellular pH (pHᵢ) is a fundamental regulator of numerous cellular processes, including cell growth, enzymatic activity, receptor-mediated signal transduction, ion transport, endocytosis, and cell adhesion. Dysregulated pHᵢ is associated with a variety of diseases, highlighting the need for reliable tools to measure dynamic pH changes in live cells.
ION Biosciences’ Brilliant Intracellular pH Assay is a high-throughput, no-wash, fluorescence-based platform optimized to monitor activation of plasma membrane proton-coupled channels and transporters in response to pharmacological compounds. These include the voltage-gated proton channel (HV1), Na+/H+ exchangers (SLC9), divalent metal
transporters (SLC11), peptide transporters (SLC15), monocarboxylate transporters (SLC16), proton-coupled amino acid transporters (SLC36), proton-coupled sugar transporters (SLC45), and the proton-coupled folate transporter (SLC46).
The assay uses the cell-permeable dye BCECF AM, a green fluorescent pH indicator with a pKa of ~7 and dual-excitation properties (Ex/Em ~430/535 nm and ~490/535 nm). The ratio of these emission intensities is directly proportional to the intracellular pH and can be used to quantify co-transport of other substrates in proton-coupled SLC transporters. Ratiometric measurement enables precise determination of absolute pHᵢ while minimizing variability from photobleaching, dye loading, or cell morphology. For high-throughput screening, BCECF can also be measured non-ratiometrically using standard fluorescein settings. The assay delivers sensitive, quantitative readouts with strong signal and broad dynamic range, compatible with 96- and 384-well plates, making it ideal for mechanistic studies, dose–response profiling, and large-scale screening campaigns.
Measure the activity of proton-dependent SLC transporters like PepT1 in response to substrate addition in realtime, and quantify important compound effects such as substrate affinity.
Identify if compounds are substrates or modulators for specific SLC transporters or H+ channels, like TMEM175.
Use intracellular pH calibration solutions to correlate readouts to specific intracellular pH values.
