SPECIFICATIONS
Excitation:
340 
380 nm 
Emission:
505 nm 
Kd:
145 nM
MW:
1002
Solubility:
DMSO
Purity:
>95%
Product code: 6000-10
product description
Ratiometric Calcium Essentials kit provides the necessary reagents for conducting no wash, ratiometric calcium flux assays compatible with plate reader and fluorescence microscopy applications. Individual components are provided to give the user the flexibility needed to customize your assay.
Fura-2 is the most popular UV-excitable, ratiometric green indicator for intracellular calcium (Ca²⁺) measurements. Ex/Em: 340/505 nm can be used to measure Ca²⁺-bound Fura-2, and Ex/Em: 380/505 nm can be used to detect Ca²⁺-free Fura-2.
Ratiometry is not only optimal for imaging applications where quantification of intracellular Ca²⁺ concentrations is desired, it also reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.
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