Custom cell lines made for high throughput screening
Every high throughput screening assay starts with a cell line expressing, or overexpressing, your protein of interest. At ION, we specialize in engineering custom cells to stably overexpress membrane bound proteins including ion channels, transporters, and GPCRs for drug discovery campaigns. We employ a variety of gene expression tools including BacMam vectors for just in time expression, as well as plasmid and lentiviral-based constructs for stable expression. From vector design to functional validation, we ensure your cells are fit for purpose.
What sets us apart is that we select clones based on functional performance in a HTS assay. We can provide polyclonal cell populations or monoclonal cell lines and include validated assay protocols for your hit finding campaign. Our standard timelines for polyclonal and monoclonal cell lines are below:
