Fluo-3 K⁺ Salt

$161.00

Green fluorescent, calcium (Ca²⁺) indicator, membrane impermeable.

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Variation	SKU	Price	Quantity	Action
Size: 1 mg	1032B	$161.00	Fluo-3 K⁺ Salt quantity	Add to cart
Size: 20 x 50 ug	1032E	$161.00	Fluo-3 K⁺ Salt quantity	Add to cart

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SKU: 1032 Categories: Calcium, Ion indicators Synonyms: fluo, fluo 3 k, fluo k, fluo salt, fluo-3, variable

Specifications

Excitation: 505 nm

Emission: 525 nm

Solubility: H2O

Purity: >90%

Storage: -20°C

Product Description

Fluo-3 is a green fluorescent, intracellular calcium (Ca²⁺) indicator with slightly red-shifted spectral properties compared to Fluo-4. Offers a large dynamic range that can be better suited for high contrast imaging applications. Is preferred over Fluo-4 for some cell types.

Fluo-3 K⁺ salt is a membrane impermeable form of Fluo-3 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.

Documentation

Learn more about Calcium indicators and assays

References

Minta A, Kao JP, Tsien RY. Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores. J Biol Chem. 1989 May 15;264(14):8171-8

Hagen BM, Boyman L, Kao JP, Lederer WJ. A comparative assessment of fluo Ca2+ indicators in rat ventricular myocytes. Cell Calcium. 2012 Aug;52(2):170-81. doi: 10.1016/j.ceca.2012.05.010

Thomas D, Tovey SC, Collins TJ, Bootman MD, Berridge MJ, Lipp P. A comparison of fluorescent Ca2+ indicator properties and their use in measuring elementary and global Ca2+ signals. Cell Calcium. 2000 Oct;28(4):213-23. doi: 10.1054/ceca.2000.0152

Donnadieu E, Bourguignon LY. Ca2+ signaling in endothelial cells stimulated by bradykinin: Ca2+ measurement in the mitochondria and the cytosol by confocal microscopy. Cell Calcium. 1996 Jul;20(1):53-61. doi: 10.1016/s0143-4160(96)90050-0

Zixiu Xiang 1, Analisa D Thompson, John T Brogan, Michael L Schulte, Bruce J Melancon, Debbie Mi, L Michelle Lewis, Bende Zou, Liya Yang, Ryan Morrison, Tammy Santomango, Frank Byers, Katrina Brewer, Jonathan S Aldrich, Haibo Yu, Eric S Dawson, Min Li, Owen McManus, Carrie K Jones, J Scott Daniels, Corey R Hopkins, Ximin Simon Xie, P Jeffrey Conn, C David Weaver, Craig W Lindsley. The Discovery and Characterization of ML218: A Novel, Centrally Active T-Type Calcium Channel Inhibitor with Robust Effects in STN Neurons and in a Rodent Model of Parkinson’s Disease. ACS Chem Neurosci. 2011. 21;2(12):730-742. doi: 10.10

Loughrey CM, MacEachern KE, Cooper J, Smith GL. Measurement of the dissociation constant of Fluo-3 for Ca2+ in isolated rabbit cardiomyocytes using Ca2+ wave characteristics. Cell Calcium. 2003 Jul;34(1):1-9. doi: 10.1016/s0143-4160(03)00012-

Vandenberghe PA, Ceuppens JL. Flow cytometric measurement of cytoplasmic free calcium in human peripheral blood T lymphocytes with fluo-3, a new fluorescent calcium indicator. J Immunol Methods. 1990 Mar 9;127(2):197-205. doi: 10.1016/0022-1759(90)90069-8

Schepers E, Glorieux G, Dhondt A, Leybaert L, Vanholder R. Flow cytometric calcium flux assay: evaluation of cytoplasmic calcium kinetics in whole blood leukocytes. J Immunol Methods. 2009 Aug 31;348(1-2):74-82. doi: 10.1016/j.jim.2009.07.002

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