Fluo-3 AM

$82.00 – $181.00

Fluo-3 AM is a green fluorescent, calcium indicator. Membrane-permeable.

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Fluo-3 AM calcium indicator packaging, red background

Fluo-3 AM calcium indicator packaging, transparent background

SKU: 1031 Categories: Ion indicators, Calcium Synonyms: fluo, fluo-3, variable

Specifications

Excitation: 505 nm

Emission: 525 nm

Solubility: DMSO

Purity: >95%

Storage: -20°C

Size: 1 mg, 20 x 50 ug, 5 x 50 ug

CAS Number: 121714-22-5

Product Description

Fluo-3 is a green fluorescent, intracellular calcium (Ca²⁺) indicator with slightly red-shifted spectral properties compared to Fluo-4. Offers a large dynamic range that can be better suited for high contrast imaging applications. Is preferred over Fluo-4 for some cell types.

Documentation

Learn more about Calcium indicators and assays

References

Minta A, Kao JP, Tsien RY. Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores. J Biol Chem. 1989 May 15;264(14):8171-8

Hagen BM, Boyman L, Kao JP, Lederer WJ. A comparative assessment of fluo Ca2+ indicators in rat ventricular myocytes. Cell Calcium. 2012 Aug;52(2):170-81. doi: 10.1016/j.ceca.2012.05.010

Thomas D, Tovey SC, Collins TJ, Bootman MD, Berridge MJ, Lipp P. A comparison of fluorescent Ca2+ indicator properties and their use in measuring elementary and global Ca2+ signals. Cell Calcium. 2000 Oct;28(4):213-23. doi: 10.1054/ceca.2000.0152

Donnadieu E, Bourguignon LY. Ca2+ signaling in endothelial cells stimulated by bradykinin: Ca2+ measurement in the mitochondria and the cytosol by confocal microscopy. Cell Calcium. 1996 Jul;20(1):53-61. doi: 10.1016/s0143-4160(96)90050-0

Loughrey CM, MacEachern KE, Cooper J, Smith GL. Measurement of the dissociation constant of Fluo-3 for Ca2+ in isolated rabbit cardiomyocytes using Ca2+ wave characteristics. Cell Calcium. 2003 Jul;34(1):1-9. doi: 10.1016/s0143-4160(03)00012-

Vandenberghe PA, Ceuppens JL. Flow cytometric measurement of cytoplasmic free calcium in human peripheral blood T lymphocytes with fluo-3, a new fluorescent calcium indicator. J Immunol Methods. 1990 Mar 9;127(2):197-205. doi: 10.1016/0022-1759(90)90069-8

Schepers E, Glorieux G, Dhondt A, Leybaert . L, Vanholder R. Flow cytometric calcium flux assay: evaluation of cytoplasmic calcium kinetics in whole blood leukocytes. J Immunol Methods. 2009 Aug 31;348(1-2):74-82. doi: 10.1016/j.jim.2009.07.002

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