NHE1 Assay(s)

Background Information

Electroneutral Na⁺/H⁺ exchanger 1 maintains intracellular pH, cell volume, and cytoskeletal anchoring. Critical mediator of pathological intracellular Na⁺ and secondary Ca²⁺ overload during myocardial ischemia-reperfusion injury, contributing to infarction, contracture, and arrhythmias. In oncology, NHE1 is upregulated across solid tumors, extruding protons to create an acidic extracellular microenvironment that drives tumor invasion and chemoresistance.

Family: SLC Transporter
Protein Name: NHE1 (Endogenous)
Gene Name: SLC9A1

Organism : Human
Therapeutic Area: Cardiovascular, Oncology, Neurodegeneration

Assay Information

Validated Assay(s): Sodium-dependent SLC Transporter Assay, Intracellular pH Assay
Assay type: Cell-based
Method: Fluorescence

Instrumentation: Panoptic
Plate Format: 384-well
Expression: Endogenous
Cell type: HEK293T

Testing Information

Functional Mode(s): Inhibitor
Reference compound(s): EIPA

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