NaCT Assay(s)

Background Information

The sodium-coupled citrate transporter (NaCT), encoded by the SLC13A5 gene, mediates sodium-dependent citrate uptake across the plasma membrane. NaCT plays an important role in citrate transport and cellular metabolism, making it a relevant target for studying transporter biology and screening compounds that modulate citrate transport activity.

Family: SLC Transporter
Protein Name: NaCT
Gene Name: SLC13A5

Organism : Human
Therapeutic Area: Metabolic

Assay Information

Validated Assay(s): Sodium-dependent SLC Transporter Assay
Assay type: Cell-based
Method: Fluorescence

Instrumentation: TBD
Plate Format: TBD
Expression:TBD
Cell type: HEK T

Testing Information

Functional Mode(s): Substrate uptake / transporter activity
Test Substrate: Citrate
Comparator: Pyruvate
Control Cells: Wildtype HEK T
Reported EC50: 0.398 mM

NaCT-expressing HEK T cells showed a robust citrate-dependent response, while wildtype HEK T cells showed minimal activity. Comparative testing with citrate and pyruvate supported assay specificity for NaCT-mediated citrate transport.

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