Sodium-dependent glycine transporter 1 (GlyT1, encoded by SLC6A9) is a transmembrane transporter responsible for the reuptake of glycine, the major inhibitory neurotransmitter in the central nervous system. GlyT1 is a member of the SLC6 family of sodium- and chloride-dependent neurotransmitter transporters. The transporter plays a key role in regulating extracellular glycine concentrations, particularly in glycinergic synapses and in modulating N-methyl-D-aspartate (NMDA) receptor activity through glycine clearance. Dysregulation of GlyT1 function has been implicated in various neurological and psychiatric disorders, including schizophrenia, epilepsy, and cognitive dysfunction. As such, GlyT1 is a promising pharmacological target for the development of novel therapeutics aimed at modulating glycinergic and glutamatergic neurotransmission.