The dopamine transporter (DAT), encoded by the SLC6A3 gene, is a sodium- and chloride-dependent symporter responsible for the reuptake of dopamine from the synaptic cleft into presynaptic neurons. By tightly regulating extracellular dopamine levels, DAT plays a critical role in modulating dopaminergic signaling in the central nervous system, particularly within regions such as the striatum, nucleus accumbens, and prefrontal cortex. Dysregulation of DAT function has been implicated in a variety of neurological and psychiatric disorders, including Parkinson’s disease, attention-deficit/hyperactivity disorder (ADHD), bipolar disorder, and substance use disorders.