PTH1R Assay(s)

Background Information

Dual Gq/Gs-coupled PTH receptor regulating calcium/phosphate homeostasis, renal calcium reabsorption, and bone turnover. Target for anabolic osteoporosis therapy (PTH analogs stimulate bone formation) and hypoparathyroidism. Constitutively activating mutations cause Jansen metaphyseal chondrodysplasia; inactivating mutations cause Blomstrand lethal chondrodysplasia.

Family: GPCR
Protein Name: PTH1 Receptor
Gene Name: PTH1R

Organism: Human
Therapeutic Area: Metabolic, Rare-disease

Assay Information

Validated Assay(s): cAMP, Calcium Assay
Assay type: Cell-based
Method: Fluorescence

Instrumentation: FLIPR, Panoptic
Plate Format: 384-well
Expression: Transient
Cell type: HEK293T

Testing Information

Functional Mode(s): Agonist, Antagonist
Reference compound(s): PTH(1-34)

PTH(1-34) concentration-response data for the human PTH1R receptor using cAMP and calcium fluorescence assays in HEK293T cells.
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