MRGPRX2 Assay(s)

Background Information

Gq-coupled GPCR mediating mast cell pseudoallergy (complement-independent, IgE-independent degranulation). Expressed on peripheral sensory neurons and lung mast cells. Mediates adverse drug reactions to opioids (morphine, codeine), neuromuscular blockers (rocuronium), fluoroquinolones, and contrast agents. Key target at the pain-inflammation interface.

Family: GPCR
Protein Name: MRGPRX2
Gene Name: MRGPRX2

Organism: Human
Therapeutic Area: Pain, Immunology, Respiratory

Assay Information

Validated Assay(s): Calcium Assay
Assay type: Cell-based
Method: Fluorescence

Instrumentation: FLIPR
Plate Format: 384-well
Expression: Transient
Cell type: HEK293T

Testing Information

Functional Mode(s): Agonist, Antagonist
Reference compound(s): Cortistatin-14

Cortistatin concentration-response curve for the human MRGPRX2 receptor in a Brilliant Calcium fluorescence assay using HEK293T cells.
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