ION assays and reagents are manufactured under rigorous quality standards to ensure consistent performance and minimize lot-to-lot variability.
High-quality raw materials carefully selected and tested.
Assays produced under strict conditions to ensure consistency.
Each lot undergoes extensive quality control testing.
Functional performance is validated in relevant biological assays.
Only lots that meet our rigorous acceptance criteria are released.
You receive a lot-tested product you can trust in your research.
We test every lot to ensure consistent performance and low lot-to-lot variability.
Below is an example of four lots of the same product tested over four different years.
Consistent product fill weight across 4 lots manufactured over 4 years.
Equivalent performance across 4 lots in a cell-based assay
ION products are used daily in our Assay Services
department to generate high-quality in-house data.
Our scientists depend on the same reliable
performance as our customers do.
Reliable results across experiments and over time.
Minimized lot-to-lot variability for more dependable data.
High-quality data you can trust for critical decisions.
Focus on discovery with confidence in your assay.
For fluorescent dyes, ION’s quality control team generally performs structural confirmation by 1H-NMR, mass confirmation and purity determination by LC/MS, absorbance maxima by ultraviolet spectrophotometry, fluorescence excitation and emission maxima by spectrofluorimetry, and ‘bio-validation’ in an appropriate cell assay. For buffer solutions, physical appearance, pH and osmolality are tested prior to subjecting each buffer lot to an appropriate ‘bio-validation,’ further ensuring each product from ION Biosciences effectively supports your fluorescence-based assays.
‘Bio-validation’ is the phrase we use to describe a cell-based assay that each of our dyes is subjected to for product certification, before release. Effectively, each lot of dye is loaded into engineered cells to evaluate the change in fluorescence (F/F0) as the dye responds to cellular stimuli. Intracellular dyes (the acetoxymethyl ester variants) are bio-validated intracellularly, and water-soluble salt variants of dyes are qualified in in vitro assays.
Certificates of Analysis can be found on each product page, by clicking the ‘Documentation’ button at the top or by scrolling down to the bottom of each page and clicking the blue ‘COA’ icon, and selecting the appropriate lot number to retrieve the certificate.
We simply want to provide assurance to our customers that every lot is of the highest quality. By consistently producing the highest quality products, we ensure that your lot-to-lot variability is minimal, so assays are conducted with as much confidence as the frontiers of science can provide.
By conducting cell validations of each dye and buffer lot, you can trust that ION Biosciences has made every attempt to carefully characterize our products, and ensure that confidence in ION's quality before your research.
Depending on the product type, validations may include cell-based assays, in vivo assays to confirm target response (e.g., Kd), or other functional tests that demonstrate consistent performance.
These tests ensure our products perform as expected before every lot is released.
Talk directly with our scientists about your research needs.
