Fura-2 AM

Name: Fura-2 AM | Ratiometric Calcium Indicator | ION Biosciences
SKU: 1051A
Availability: InStock

Price range: $115.00 through $460.00

UV-excitable, ratiometric green calcium (Ca²⁺) indicator, membrane permeable.

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Variation	SKU	Price	Quantity	Action
Size: 1 mg	1051B	$115.00	Fura-2 AM quantity	Add to cart
Size: 5 mg	1061A	$460.00	Fura-2 AM quantity	Add to cart
Size: 20 x 50 ug	1051E	$115.00	Fura-2 AM quantity	Add to cart

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Fura-2 AM ratiometric calcium indicator packaging, red background

Fura-2 AM ratiometric calcium indicator packaging, transparent background

SKU: 1051A Categories: Calcium, Ion indicators Synonyms: fura, fura 2, variable

Specifications

Excitation: 340 nm, 380 nm

Emission: 505 nm

Kd: 145 nM

MW: 1002 g/mol

Solubility: DMSO

Purity: >95%

Filter Set: Fura-2

Storage: -20°C

Size: 1 mg, 20 x 50 ug, 5 mg

CAS Number: 108964-32-5

Product Description

Fura-2 is the most popular UV-excitable, ratiometric green indicator for intracellular calcium (Ca²⁺) measurements. Ex/Em: 340/505 nm can be used to measure Ca²⁺-bound Fura-2, and Ex/Em: 380/505 nm can be used to detect Ca²⁺-free Fura-2. Ratiometry is optimal for imaging applications where quantification of intracellular Ca²⁺ concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.

Sample data showing kinetic fluorescence data of fura-2 AM (bound and unbound) in response to calcium influx with the corresponding fluorescence ratio plotted separately.

Ratiometric images of Fura-2 AM loaded cells before and after the addition of ionomycin, which causes an influx of calcium into the cells that's reported as a higher ratio of bound/unbound Fura-2 fluorescence.

GPCR assay using Fura-2 AM to measure calcium flux. Dose dependent response of carbachol stimulation of the endogenous muscarinic M3 receptor in HEK cells.

Documentation

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