MrgD Assay(s)

Background Information

Mas-related GPCR expressed on a distinct subset of unmyelinated C-fiber sensory neurons in dorsal root ganglia and skin. Primary endogenous agonist: β-alanine. Mediates histamine-independent itch transmission; genetic ablation of MrgD-positive neurons in mice abolishes dry skin-induced pruritus. Distinct from the classic H1R-mediated itch pathway. Early preclinical interest for chronic pruritus and atopic dermatitis.

Family: GPCR
Protein Name: MRGPRD
Gene Name: MRGPRD

Organism: Human
Therapeutic Area: Pain, Immunology

Assay Information

Validated Assay(s): cAMP, Calcium Assay
Assay type: Cell-based
Method: Fluorescence

Instrumentation: Panoptic
Plate Format: 384-well
Expression: Stable
Cell type: HEK293T

Testing Information

Functional Mode(s): Agonist, Antagonist
Reference compound(s): b-alanine

β-Alanine concentration-response curve for the human MrgD receptor in a fluorescence-based GPCR assay using HEK293T cells.
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