ION VITAL – MitoVolt


Mitochondrial membrane potential assay kit that uses JC-10, a dye that accumulates in polarized mitochondria where it emits a red-shifted fluorescence, that is more soluble than JC-1. 5 plates


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490, 540 nm


525, 590 nm


~600 g/mol





Product code: 5100. *This product is not compatible with fixation.

product description

Fluorescence images of JC-10 stained cells acquired before and after FCCP treatment.

ION Vital – MitoVolt assay kit is the ideal solution for detecting changes in mitochondrial membrane potential due to cell apoptosis or other stress-inducing phenomena. ION Vital – MitoVolt is compatible with fluorescence microscopy, flow cytometry, and plate reader applications.

Data showing loss of mitochondrial activity, or mitochondrial membrane potential, in response to staurosporine and CCCP-treated cells using ION Vital - MitoVolt.

JC-10 is a mitochondrial membrane potential probe. It possesses superior aqueous solubility compared to its better known analogue, JC-1. At low concentrations, JC-10 is monomeric and emits a green fluorescence. JC-10 accumulates in healthy mitochondria, forming J-aggregates that exhibit an orange fluorescence. Mitochondrial depolarization, a key marker of cellular apoptosis, results in a loss of JC-10 accumulation and a reversal to its monomeric form. This reversible behavior of JC-10 allows for ratiometric analysis of mitochondrial membrane potential, where a shift from orange (Ex/Em: 540nm/590nm) to green fluorescence (Ex/Em: 490nm/525nm) is indicative of compromised mitochondria.

ION Vital – MitoVolt can be used to monitor and/or visualize mitochondrial membrane potential as a static endpoint or in real-time. When following our protocol, ION Vital – MitoVolt provides enough reagents to make 25 mL of working solution, enough for five 96-well plates or 500 flow cytometry samples. The actual number of assays will vary according to optimal dye concentrations and assay volumes for your application.