$695.00 – $11,690.00
A complete Gi/o GPCR assay for discovering modulators through direct coupling to GIRK channels. Cell lines and thallium flux reagents are included.
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| Variation | SKU | Price | Quantity | Action |
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Size: Flex kit - 10 plate without cells
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11100-b
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$695.00
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Size: Flex kit - 10 plate with cells
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11100-a
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$11,690.00
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ION Assurance – We guarantee product quality. If you’re unsatisfied with your order, send us an email at support@ionbiosciences.com. We’ll promptly address your concern and replace, credit, or refund your items according to our ION Assurance terms and conditions.
ION’s Gi/o GPCR-GIRK Thallium Flux Assay is a total assay solution for multi-well plate-based, high-throughput measurements of Gi/o GPCR agonism measured via native GIRK coupling. This kit provides most reagents needed, including a stable HEK293 cell line that expresses human Kir3.1 and Kir3.2, to conduct Gi/o GPCR HTS. A simple transduction step allows the flexibility to study a plethora of GPCRs that couple to GIRK using thallium flux.
Key Benefits:
Insert any Gi/o GPCR of interests using transient or stable expression systems for rapid hit finding. Generate HTS results for your target within 24 hours.
As demonstrated in this dopamine (D2) receptor example, cAMP assays can be complicated by the combination of Gs and Gi GPCR receptor expression. HEK cells endogenously express multiple dopamine receptors, Our Gi/o GPCR-GIRK Thallium Flux Assay readout is specific to Gi GPCR signaling, enabling you to ignore the noise and hone in on your target of interest.
Unlock better assay quality with Z’ out-matching cAMP assays. When studying Gi-coupled receptors which inhibit cAMP production, the high basal cAMP levels induced by forskolin can make it difficult to detect small decreases in cAMP caused by receptor activation.
New to thallium flux assays, read our blog called How to analyze thallium flux assay data.
