$430.00 – $1,047.00
Thallium-free GIRK Potassium Channel Assay is the first fluorescence-based potassium channel solution that directly measures potassium flux, enabling you to move away from thallium-containing products. This product features the potassium-sensitive dye, IPG-1 AM, our lowest affinity potassium indicator to measure changes in intracellular potassium concentration. This kit is optimized for GIRK (Kir3.x) screening, and can be used to identify activators and inhibitors of GIRK channels.
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Traditional approaches for measuring GIRK activity such as patch clamp, FRET, or thallium flux assays can be labor-intensive, low-throughput, or reliant on toxic surrogate ions. To overcome these limitations, ION Biosciences built the Thallium-Free GIRK Potassium Channel Assay featuring ION Potassium Green-1 AM (IPG-1 AM), a membrane-permeable, potassium-sensitive, fluorescent indicator that directly measures potassium flux.
Upon binding of an agonist to Gi/o-coupled GPCRs or direct activation of GIRK channels, potassium ions flow through the channel and out of the cell, resulting in a measurable decrease in intracellular potassium concentration. This change in potassium concentration is detected in real-time by IPG-1 as a loss of fluorescence intensity. This direct-readout eliminates the need for surrogate ions or radioactive tracers and enables robust, high-throughput screening to identify GIRK modulators in 96- and 384-well formats.
The Thallium-Free GIRK Potassium Channel Assay provides reliable EC50/IC50 values and is ideally suited for pharmacological profiling and discovery of therapeutic compounds targeting GIRK channels or GIRK channel-coupled pathways.
This kit has not been validated for use with additional potassium channel targets at this time.
All data displayed was generated using our stable HEK293 – huKir3.1/3.2 cell line.
Identify activators: Compounds that increase GIRK activity, such as ML297, exhibit a concentration dependent loss of intracellular fluorescence, indicative of K+ efflux.
Identify inhibitors: Compounds that inhibit GIRK activity, such as SCH23390, do not display a loss of fluorescence upon treatment with a potent activator such as ML297.
Large screening windows in activator and inhibitor modes with Z’ > 0.8 enable reliable hit-finding campaigns.
