$380.00
Ratiometric potassium indicator, membrane-impermeable.
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Size: 1 mg
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3033B
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$380.00
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Size: 20 x 50 ug
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3033E
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$380.00
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Emission: 505 nm
Kd: 5 mM
MW: 1175 g/mol
Solubility: H2O
Purity: >90%
Filter Set: Fura-2
Storage: -20°C
CAS Number: 124549-11-7
PBFI is a UV-excitable, ratiometric green indicator for intracellular potassium (K⁺) measurements. Ex/Em: 340/505 nm can be used to measure K⁺-bound PBFI, and Ex/Em: 380/505 nm can be used to detect K⁺-free PBFI. It is 1.5X more selective for K⁺ over Na⁺and works well in cytosolic conditions where [K⁺]/[Na⁺] is ~10. Ratiometry is optimal for imaging applications where quantification of intracellular K⁺ concentrations is desired and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.
PBFI K⁺ salt is a membrane impermeable form of PBFI that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection, or other methods.
