Fluo-4 AM

$71.00 – $154.00

Fluo-4 is a green fluorescent, calcium indicator. Membrane-permeable. A popular solution for high-throughput calcium flux assays and GPCR assays.

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Fluo-4 AM calcium indicator packaging, red background

Fluo-4 AM - Image 2

SKU: 1041 Categories: Calcium, Ion indicators Synonyms: Cal 520 AM, CalBryte 520 AM, fluo 4, fluo-4 am, Fluo-8 AM, variable

Specifications

Excitation: 490 nm

Emission: 515 nm

Solubility: DMSO

Purity: >95%

Filter Set: FITC, GFP

Storage: -20°C

Size: 10 x 50 ug, 3 x 50 ug, 500 ug

CAS Number: 273221-67-3

Product Description

Fluo-4 is the most popular green fluorescent, intracellular calcium (Ca²⁺) indicator. Proven and trusted for decades to measure intracellular Ca²⁺ flux in high throughput screening and fluorescence microscopy applications.

Fluorescence images of Fluo-4 stained cells before and after the addition of ionomycin

Documentation

Learn more about Calcium indicators and assays

References

Horning, K. J., Joshi, P., Nitin, R., Balachandran, R. C., Yanko, F. M., Kim, K., … & Bowman, A. B. (2020). Identification of a selective manganese ionophore that enables nonlethal quantification of cellular manganese. Journal of Biological Chemistry, 295(12), 3875-3890

Gee KR, Brown KA, Chen WN, Bishop-Stewart J, Gray D, Johnson I. Chemical and physiological characterization of fluo-4 Ca(2+)-indicator dyes. Cell Calcium. 2000 Feb;27(2):97-106. doi: 10.1054/ceca.1999.0095

Bovo E, Dvornikov AV, Mazurek SR, de Tombe PP, Zima AV. Mechanisms of Ca²+ handling in zebrafish ventricular myocytes. Pflugers Arch. 2013 Dec;465(12):1775-84. doi: 10.1007/s00424-013-1312-2. Epub 2013 Jul 3. PMID: 23821298; PMCID: PMC4138713.

Rodriguez AL, Grier MD, Jones CK, Herman EJ, Kane AS, Smith RL, Williams R, Zhou Y, Marlo JE, Days EL, Blatt TN, Jadhav S, Menon UN, Vinson PN, Rook JM, Stauffer SR, Niswender CM, Lindsley CW, Weaver CD, Conn PJ. Discovery of novel allosteric modulators of metabotropic glutamate receptor subtype 5 reveals chemical and functional diversity and in vivo activity in rat behavioral models of anxiolytic and antipsychotic activity. Mol Pharmacol. 2010. 78(6):1105-23.

Zixiu Xiang 1, Analisa D Thompson, John T Brogan, Michael L Schulte, Bruce J Melancon, Debbie Mi, L Michelle Lewis, Bende Zou, Liya Yang, Ryan Morrison, Tammy Santomango, Frank Byers, Katrina Brewer, Jonathan S Aldrich, Haibo Yu, Eric S Dawson, Min Li, Owen McManus, Carrie K Jones, J Scott Daniels, Corey R Hopkins, Ximin Simon Xie, P Jeffrey Conn, C David Weaver, Craig W Lindsley. The Discovery and Characterization of ML218: A Novel, Centrally Active T-Type Calcium Channel Inhibitor with Robust Effects in STN Neurons and in a Rodent Model of Parkinson’s Disease. ACS Chem Neurosci. 2011. 21;2(12):730-742. doi: 10.1021/cn200090z.

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