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Calcein AM is a membrane-permeant, non-fluorescent indicator that is converted to green fluorescent calcein by esterase activity in viable cells.
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Excitation: 495 nm
Emission: 515 nm
MW: 995 g/mol
Solubility: DMSO
Purity: >95%
Filter Set: FITC
Storage: -20°C
Size: 1 mg, 2 mM in DMSO, 0.5 mL, 20 x 50 ug
CAS Number: 148504-34-1
Calcein AM is a membrane-permeant, non-fluorescent form of calcein that is converted to green fluorescent calcein in viable cells, resulting in uniform cytosolic fluorescence (Ex/Em 495 nm/515 nm). Calcein is well retained within the cytosol of most healthy cells with intact cell membranes, and is most commonly used as an indicator of cell viability.