$228.00 – $3,623.00
Brilliant Sodium is the first high-throughput, no wash sodium channel assay designed to measure changes in intracellular sodium caused by effectors of sodium channels, sodium transporters, and non-selective cation channels.
Showing 1 - 3 out of 3
Page 1 out of 1
| Variation | SKU | Price | Quantity | Action |
|---|---|---|---|---|
|
Size: pIONeer Kit - 2 plates
|
9000-2
|
$228.00
|
|
|
|
Size: Flex Kit - 10 plates
|
9000-10
|
$478.00
|
|
|
|
Size: Express Kit - 100 plates
|
9000-100
|
$3,623.00
|
|
ION Assurance – We guarantee product quality. If you’re unsatisfied with your order, send us an email at support@ionbiosciences.com. We’ll promptly address your concern and replace, credit, or refund your items according to our ION Assurance terms and conditions.
ION’s Brilliant Sodium Assay is a total assay solution for multi-well plate-based, high-throughput measurements of changes in intracellular Na⁺ mediated through a wide-variety of plasma membrane and intracellular sodium channels and transporters. In multi-well, plate-based formats, the Brilliant Sodium Assay can be used to discover and characterize the effects of many tens-of-thousands of compounds and environmental factors on effectors of intracellular Na⁺. ION’s Brilliant Sodium Assay provides all the reagents necessary for use as a wash or no-wash assay with adherent or non-adherent cells. The optional use of a probenecid solution and an extracellular background masking solution (TRS) offers the ultimate in compatibility for cells types which are difficult to load with fluorescent Na⁺ indicators (e.g. Chinese Hamster Ovary, CHO cells) and when performing assays in complete, serum-containing cell culture medium is desired.
ION’s Brilliant Sodium Assay is compatible with fluorescence microscopes, flow cytometers, and plate readers capable of detecting fluorescein or more optimally, yellow fluorescent protein (YFP).
Our Flex kit provides all necessary reagents in a format to maximize flexibility for your assay design and customization challenges. If you want a kit designed just for you, inquire about our Brilliant Sodium Express Kit.
Key Benefits:
Measuring the native ion ensures you:
Compatible with more instruments:
Works for more than voltage-gated sodium channels:
Patch clamp versus Brilliant Sodium
A small library screen (78 compounds) for Nav1.X inhibitors confirms accurate hit identification and strong IC50 correlation between Brilliant Sodium assay (ING-2) and the IonWorks Barracuda automated patch clamp system (EP). Dose response curves of 3 separate hits are also displayed.
Brilliant Sodium vs. Membrane Potential Assays
Measuring the native ion instead of a surrogate can help you find more real hits and substantially reduce false positives in your HTS campaign. A screen (27,978 compounds) for Nav1.X inhibitors shows a 10% reduction in false positives with our sodium-selective assay when compared to FLIPR membrane potential.
Assay Nav1.1 – Nav1.8
Use Brilliant Sodium to discover modulators for all voltage-gated sodium channels (Nav1.x).
Works with iPSCs
Screen for Nav activity with endogenous expression levels in iPSC-derived sensory neurons. Distinguish between TTX-resistant and TTX-sensitive sodium channels.
Endpoint compatible
Acquire exceptional data on almost any fluorescence-capable instrument, without the need for automation. Data shown is for a Nav1.3 inhibition assay.
Works for additional targets too
Brilliant Sodium be used to measure the activity of transporters like the sodium/potassium pump, SLC transporters, ligand-gated channels, and TRP channels as well.
See how simple it is to incorporate Brilliant Sodium Assays into your workflow by watching this short how-to video for screening Nav1.5.
Review our detailed application note that walks you through the essential steps in conducting a high-throughput Nav1.5 sodium channel assay.
Working with complex co-culture systems? That’s not a problem for Brilliant Sodium! Read our blog post detailing how to acquire cell-specific CRCs from individual cell types in mixed cultures.
