SBFI K⁺ SALT

$360.00

Ratiometric sodium (Na⁺) indicator, membrane impermeable

 

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SPECIFICATIONS

Excitation:

340   380 nm 

Emission:

505 nm 

Kd:

4 mM

MW:

991

Solubility:

H2O

Synonyms: Sodium-binding benzofuran isophthalate – AM, sodium binding indicator
Product code: 1mg: 2022B/ 20 x 50mg: 2022E

product description

SBFI is a UV-excitable, ratiometric green indicator for intracellular sodium (Na⁺) measurements. Ex/Em: 340/505 nm can be used to measure Na⁺-bound SBFI, and Ex/Em: 380/505 nm can be used to detect Na⁺-free SBFI. It is ~18X more selective for Na⁺ over K⁺. Ratiometry is optimal for imaging applications where quantification of intracellular Na⁺ concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.

SBFI K⁺ salt is a membrane impermeable form of SBFI that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.

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