340 380 nm
Synonyms: Sodium-binding benzofuran isophthalate – AM, sodium binding indicator
Product code: 1mg: 2022B/ 20 x 50mg: 2022E
SBFI is a UV-excitable, ratiometric green indicator for intracellular sodium (Na⁺) measurements. Ex/Em: 340/505 nm can be used to measure Na⁺-bound SBFI, and Ex/Em: 380/505 nm can be used to detect Na⁺-free SBFI. It is ~18X more selective for Na⁺ over K⁺. Ratiometry is optimal for imaging applications where quantification of intracellular Na⁺ concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.
SBFI K⁺ salt is a membrane impermeable form of SBFI that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.