Synonyms: ION Natrium Green – 2, Asante Natrium Green – 2 (ANG-2)
Product code: 10 x 50ug: 2013F / 500ug: 2013C
ION Natrium Green – 2 (ING-2) is a yellow-green fluorescent, intracellular sodium (Na⁺) indicator with improved cellular loading and significantly higher brightness than SBFI. Its spectral properties (Ex/Em: 525 nm/545 nm) and large dynamic range make ING-2 the best Na⁺ indicator for high-throughput screening applications targeting Na⁺ channels, and non-selective monovalent cation channels. ING-2 has a more physiologically relevant affinity (Kd = 20 mM) than ING-1 (Kd = 92 mM). Also compatible with fluorescence microscopy using common fluorescein, GFP or more ideally YFP filters.
ING-2 TMA⁺ salt is a membrane impermeable form of ING-2 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.
Yao L, Fan P, Jiang Z, Viatchenko-Karpinski S, Wu Y, Kornyeyev D, Hirakawa R, Budas GR, Rajamani S, Shryock JC, Belardinelli L. Nav1.5-dependent persistent Na+ influx activates CaMKII in rat ventricular myocytes and N1325S mice. Am J Physiol Cell Physiol. 2011 Sep;301(3):C577-86. doi: 10.1152/ajpcell.00125.2011