Synonyms: ION Natrium Green – 2, Asante Natrium Green – 2 (ANG-2)
Product code: 10 x 50ug: 2013F / 500ug: 2013C
ION Natrium Green – 2 (ING-2) is a yellow-green fluorescent, intracellular sodium (Na⁺) indicator with improved cellular loading and significantly higher brightness than SBFI. Its spectral properties (Ex/Em: 525 nm/545 nm) and large dynamic range make ING-2 the best Na⁺ indicator for high-throughput screening applications targeting Na⁺ channels, and non-selective monovalent cation channels. ING-2 has a more physiologically relevant affinity (Kd = 20 mM) than ING-1 (Kd = 92 mM). Also compatible with fluorescence microscopy using common fluorescein, GFP or more ideally YFP filters.
ING-2 TMA⁺ salt is a membrane impermeable form of ING-2 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.
Laila Blömer, Marco Canepari, Luiza Filipis. Ultrafast Sodium Imaging of the Axon Initial Segment of Neurons in Mouse Brain Slices. Current Protocols in Bioinformatics, Wiley, 2021, 1(3). https://doi.org/10.1002/cpz1.64
Yao L, Fan P, Jiang Z, Viatchenko-Karpinski S, Wu Y, Kornyeyev D, Hirakawa R, Budas GR, Rajamani S, Shryock JC, Belardinelli L. Nav1.5-dependent persistent Na+ influx activates CaMKII in rat ventricular myocytes and N1325S mice. Am J Physiol Cell Physiol. 2011 Sep;301(3):C577-86. doi: 10.1152/ajpcell.00125.2011