340 380 nm
Synonyms: Fura-2 Leakage Resistant, Fura-PE3
Product code: 1mg: 1062B/ 20 x 50ug: 1062E
UV-excitable, ratiometric green indicator for intracellular calcium (Ca²⁺) measurements with longer cellular retention compared to Fura-2 AM and similar spectral properties. Ex/Em: 340/505 nm can be used to measure Ca²⁺-bound Fura-2, and Ex/Em: 380/505 nm can be used to detect Ca²⁺-free Fura-2. Ratiometry is optimal for imaging applications where quantification of intracellular Ca²⁺ concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.
Fura-2 LR K⁺ salt is a membrane impermeable form of Fura-2 LR that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.