Specifications
Excitation:
340 
380 nm 
Emission:
505 nm 
Kd:
145 nM
MW:
145
Solubility:
H2O
Purity:
>90%
Product code: 1mg: 1052B/ 20 x 50ug: 1052E
product description
Fura-2 is the most popular UV-excitable, ratiometric green indicator for calcium (Ca²⁺) measurements. Ex/Em: 340/505 nm can be used to measure Ca²⁺-bound Fura-2, and Ex/Em: 380/505 nm can be used to detect Ca²⁺-free Fura-2. Ratiometry is optimal for imaging applications where quantification of intracellular Ca²⁺ concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.
Fura-2 K⁺ salt is a membrane impermeable form of Fura-2 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.
documentation
