Fura-2 K⁺ Salt

$95.00

Ratiometric calcium (Ca²⁺) indicator, membrane impermeable

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Specifications

Excitation:

340  380 nm 

Emission:

505 nm 

Kd:

145 nM

MW:

145

Solubility:

H2O

Purity:

>90%

Product code: 1mg: 1052B/ 20 x 50ug: 1052E

product description

Fura-2 is the most popular UV-excitable, ratiometric green indicator for calcium (Ca²⁺) measurements. Ex/Em: 340/505 nm can be used to measure Ca²⁺-bound Fura-2, and Ex/Em: 380/505 nm can be used to detect Ca²⁺-free Fura-2. Ratiometry is optimal for imaging applications where quantification of intracellular Ca²⁺ concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.

Fura-2 K⁺ salt is a membrane impermeable form of Fura-2 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.

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