Product code: 11011-10
Brilliant Thallium Snapshot simultaneously loads cells with thallium and Thallos AM, ION’s Tl⁺-sensitive indicator, prior to adding an agonist/antagonist. The addition of an effector compound causes a change in fluorescence, due to Tl⁺ efflux or influx, that is sustained for up to 1 hr, depending on your target of interest. Brilliant Thallium Snapshot has effectively identified agonists and/or antagonists of voltage-gated potassium (hERG) and sodium (NaV) channels, inward rectifying potassium channels (GIRK1/2), and potassium and sodium transporters (Na⁺/K⁺-ATPase) using a variety of instruments.
ION Biosciences’ Brilliant Thallium (Tl⁺) Snapshot Assay is a total assay solution for multi-well plate-based, high-throughput measurements of Tl⁺ flux through K⁺, Na⁺, non-selective cation channels, and some Na⁺ or K⁺ transporters. The ION Biosciences’ Brilliant Thallium Snapshot Assay is also useful for a wide range of effectors of ion channels and transporters including G protein-coupled receptors. In multi-well, plate-based formats, the Brilliant Thallium Snapshot Assay can be used to discover and characterize the effects of tens to many tens-of-thousands of compounds and environmental factors on effectors of Tl⁺ flux. Over the past 15 years, fluorescence-based measures of Tl⁺ flux have brought about the discovery of small-molecule modulators of a host of ion channels, transporters, GPCRs, and other targets of interest for both drug discovery and basic research.