Brilliant Thallium Snapshot

$211.00$473.00

An endpoint-compatible potassium channel assay. ION’s Brilliant Thallium Snapshot kit is a thallium flux assay adaptation designed to work with standard plate readers, microscopes, flow cytometers, and more, for measuring flux through potassium (K⁺), sodium (Na⁺), and non-selective cation channels, transporters, and their effectors, including GPCRs.

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Specifications

Compatible instruments:

fluorescence microscope icon
Fluorescence Microscope
well plate icon for plate reader compatibility
Plate Reader
flow cytometry icon
Flow Cytometer
HCS (high content screening) instrument logo
HCS Instrument
HTS (high throughput screening) instrument logo
HTS Instrument

Product Description

Our patent-pending Snapshot assay format generates a long-lasting signal so you can easily detect and quantify changes in ion channel or transporter activity on most fluorescence-capable instruments. So whether you have a FLIPR, a fluorescence microscope, a standard fluorescence plate reader, a high-content imager, or a flow cytometer – you can run functional screens on viable cells using thallium flux – enabling a whole new world of possibilities.

Brilliant Thallium Snapshot simultaneously loads cells with thallium and Thallos AM, ION’s Tl⁺-sensitive indicator, prior to adding an agonist/antagonist. The addition of an effector compound causes a change in fluorescence, due to Tl⁺ efflux or influx, that is sustained for up to 1 hr, depending on your target of interest. Brilliant Thallium Snapshot has effectively identified agonists and/or antagonists of voltage-gated potassium (hERG) and sodium (NaV) channels, inward rectifying potassium channels (GIRK1/2), and potassium and sodium transporters (Na⁺/K⁺-ATPase) using a variety of instruments.

ION Biosciences’ Brilliant Thallium (Tl⁺) Snapshot Assay is a total assay solution for multi-well plate-based, high-throughput measurements of Tl⁺ flux through K⁺, Na⁺, non-selective cation channels, and some Na⁺ or K⁺ transporters. The ION Biosciences’ Brilliant Thallium Snapshot Assay is also useful for a wide range of effectors of ion channels and transporters including G protein-coupled receptors. In multi-well, plate-based formats, the Brilliant Thallium Snapshot Assay can be used to discover and characterize the effects of tens to many tens-of-thousands of compounds and environmental factors on effectors of Tl⁺ flux. Over the past 15 years, fluorescence-based measures of Tl⁺ flux have brought about the discovery of small-molecule modulators of a host of ion channels, transporters, GPCRs, and other targets of interest for both drug discovery and basic research.

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