Brilliant Thallium Gold Snapshot

Name: Brilliant Thallium Gold Snapshot | Potassium Channel Assay | ION Biosciences
SKU: 11021-1
Availability: InStock

$221.00 – $496.00

Brilliant Thallium Gold Snapshot is a multi-instrument compatible assay solution for well plate-based, high-throughput measurements of thallium flux through potassium, sodium, and non-selective cation channels, as well as some Na+ or K+ transporters. Our patent-pending Snapshot technology generates a long-lasting signal so you can easily detect and quantify changes in ion channel or transporter activity on most fluorescence-capable instruments. The inclusion of Thallos Gold AM, our red-shifted thallium indicator, makes this kit the ideal option when using GFP-expressing cells to run potassium channel assays.

Size

Clear

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Brilliant thallium gold snapshot assay kit packaging, an option for potassium channel assays, sodium channel assays, and more

SKU: 11021-1 Categories: Ion Channels and Transporter Assays, Potassium, Sodium Synonyms: Brilliant Thallium Gold Snapshot, FLIPR Potassium Assay Kit, FluoOR Red Potassium Ion Channel Assay, Fluxor II Green Potassium Ion Channel Assay, FluxOR Potassium Assay, Fluxor Potassium Ion Channel Assay, Monovalent Channel Assay Kit, Potassium Channel Assay Kit, Screen Quest™ No Wash Potassium Channel Assay, Sodium Channel Assay Kit, Thallium Assay Kit, variable

Specifications

Excitation: 530 nm

Emission: 550 nm

MW: 826 g/mol

Filter Set: YFP

Storage: Various conditions

Size: Flex Kit - 10 plates, pIONeer Kit - 2 plates

Compatible instruments:

Fluorescence Microscope

Plate Reader

Flow Cytometer

HCS Instrument

HTS Instrument

Product Description

ION Biosciences Brilliant Thallium Gold Snapshot Assay is a total assay solution for multi-well plate-based, high-throughput measurements of Tl⁺ flux through K⁺, Na⁺, non-selective cation channels, and some Na⁺ or K⁺ transporters. Our patent-pending Snapshot assay format generates a long-lasting signal so you can easily detect and quantify changes in ion channel or transporter activity on most fluorescence-capable instruments. So whether you have a FLIPR, a fluorescence microscope, a standard fluorescence plate reader, a high-content imager, or a flow cytometer – you can run functional screens on viable cells using thallium flux – enabling a whole new world of possibilities.

Snapshot Assay Principle.

Drawing that shows thallos and thallium ion loading into cells, yielding bright fluorescence

Drawing that shows thallium efflux driven by the activation of a potassium channel, resulting in a lower thallos fluorescence

Kinetic fluorescence and image data demonstrating that brilliant thallium snapshot can enable potassium channel assays on plate readers and microscopes.

hERG inhibition assay, voltage-gated potassium channel (Kv11.1) activity assay, sample data acquired using brilliant thallium gold snapshot

Brilliant Thallium Gold Snapshot simultaneously loads cells with Tl⁺ and Thallos Gold AM, ION’s Tl⁺-sensitive indicator, prior to adding an agonist/antagonist. The addition of an effector compound causes a change in fluorescence, due to Tl⁺ efflux or influx, that is sustained for up to 1 hr, depending on your target of interest. Brilliant Thallium Gold Snapshot has effectively identified agonists and/or antagonists of voltage-gated K⁺ (hERG) and Na⁺ (NaV) channels, inward rectifying K+ channels (GIRK1/2), and K⁺ and Na⁺ transporters (Na⁺/K⁺-ATPase) using a variety of instruments.