Product code: 11000-10
ION Biosciences’ Brilliant Thallium (Tl⁺) Assay is a total assay solution for multi-well plate-based, high-throughput measurements of thallium flux through potassium, sodium, non-selective cation channels, and some sodium or potassium transporters. Although thallium is primarily used as a surrogate ion for potassium channel assays, ION’s Brilliant Thallium Assay is also useful for a wide range of effectors of ion channels and transporters including G protein-coupled receptors, and lipid and protein kinases. In multi-well, plate-based formats, the Brilliant Thallium Assay can be used to discover and characterize the effects of tens to many tens-of-thousands of compounds and environmental factors on effectors of thallium flux. Over the past 15 years, fluorescence-based measures of thallium flux have been widely utilized to discover small molecule modulators of a host of ion channels, transporters, GPCRS and other targets of interest for both drug discovery and basic research.
Our Flex kit provides all necessary reagents in a format to maximize flexibility for your assay design and customization challenges.
Potassium and sodium channel assays using thallium flux
Du Y, Days E, Romaine I, Abney KK, Kaufmann K, Sulikowski G, Stauffer S, Lindsley CW, Weaver CD. Development and validation of a thallium flux-based functional assay for the sodium channel NaV1.7 and its utility for lead discovery and compound profiling. ACS Chem Neurosci. 2015 Jun 17;6(6):871-8. doi: 10.1021/acschemneuro.5b0000
Niswender CM, Johnson KA, Luo Q, Ayala JE, Kim C, Conn PJ, Weaver CD. A novel assay of Gi/o-linked G protein-coupled receptor coupling to potassium channels provides new insights into the pharmacology of the group III metabotropic glutamate receptors. Mol Pharmacol. 2008 Apr;73(4):1213-24. doi: 10.1124/mol.107.041053
Schmalhofer WA, Swensen AM, Thomas BS, Felix JP, Haedo RJ, Solly K, Kiss L, Kaczorowski GJ, Garcia ML. A pharmacologically validated, high-capacity, functional thallium flux assay for the human Ether-à-go-go related gene potassium channel. Assay Drug Dev Technol. 2010 Dec;8(6):714-26. doi: 10.1089/adt.2010.0351