SPECIFICATIONS
Excitation:
525 nm 
Emission:
545 nm 
MW:
1084
Product code: Brilliant Sodium Flex 9000-10 / Brilliant Sodium Express 9000-100
product description
ION’s Brilliant Sodium Assay is a total assay solution for multi-well plate-based, high-throughput measurements of changes in intracellular Na⁺ mediated through a wide-variety of plasma membrane and intracellular sodium channels and transporters. In multi-well, plate-based formats, the Brilliant Sodium Assay can be used to discover and characterize the effects of many tens-of-thousands of compounds and environmental factors on effectors of intracellular Na⁺. ION’s Brilliant Sodium Assay provides all the reagents necessary for use as a wash or no-wash assay with adherent or non-adherent cells. The optional use of a probenecid solution and an extracellular background masking solution (TRS) offers the ultimate in compatibility for cells types which are difficult to load with fluorescent Na⁺ indicators (e.g. Chinese Hamster Ovary, CHO cells) and when performing assays in complete, serum-containing cell culture medium is desired.
ION’s Brilliant Sodium Assay is compatible with fluorescence microscopes, flow cytometers, and plate readers capable of detecting fluorescein or more optimally, yellow fluorescent protein (YFP).
Our Flex kit provides all necessary reagents in a format to maximize flexibility for your assay design and customization challenges. If you want a kit designed just for you, inquire about our Brilliant Sodium Express Kit.
documentation
References
Laila Blömer, Marco Canepari, Luiza Filipis. Ultrafast Sodium Imaging of the Axon Initial Segment of Neurons in Mouse Brain Slices. Current Protocols in Bioinformatics, Wiley, 2021, 1 (3). https://doi.org/10.1002/cpz1.64
Tay B, Stewart TA, Davis FM, Deuis JR, Vetter I. Development of a high-throughput fluorescent no-wash sodium influx assay. PLoS One. 2019 Mar 11;14(3):e0213751.
Iamshanova, O., Mariot, P., Lehen’kyi, V. et al. Comparison of fluorescence probes for intracellular sodium imaging in prostate cancer cell lines. Eur Biophys J. 45, 765–777 (2016).
Yurinskaya VE, Aksenov ND, Moshkov AV, Goryachaya TS, Vereninov AA. Fluorometric Na+ Evaluation in Single Cells Using Flow Cytometry: Comparison with Flame Emission Assay. Cell Physiol Biochem. 2020 May 29;54(4):556-566.
Naumann G, Lippmann K, Eilers J. Photophysical properties of Na+ -indicator dyes suitable for quantitative two-photon fluorescence-lifetime measurements. J Microsc. 2018 Nov; 272(2):136-144.
