pH indicators respond to hydronium ions, and can be used to quantify solution and intracellular pH

PH Indicators

$108.00

pH Indicators

In cell biology, pH plays a crucial role in maintaining the proper functioning of cells and biological molecules. pH is generally maintained between 6.8-7.4 in the cytoplasm and in acidic organelles such as lysosomes, pH can reach as low as ~4.5. It’s also known to regulate various cellular processes, and there are a number of acid sensitive ion channels (ASICs) that modulate their activity in response to changes in pH.

The most-trusted pH indicator for tracking cellular pH is BCECF AM. BCECF has a pH-dependent ratio of emission intensity when the dye is excited at 490 nm relative to 440 nm. The ratio of these emissions is directly proportional to solution pH, and can be used to quantify pH ranges from 6 to 9. ION offers BCECF as a membrane-permeable AM ester.

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BCECF

(EX 440/490 nm, EM 535 nm) The most used intracellular pH indicator. Ratiometric readouts provide a quantitative measure of pH for cytosolic pH ranges from 6-9.

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Image	Products	Excitation	Emission	Kd	Price	Quantity	Action
	BCECF AM Ratiometric, green fluorescent pH indicator, membrane permeable.	430 nm, 490 nm	535 nm		$108.00	BCECF AM quantity	Clear Add to cart