Our best assays for measuring cell viability and mitochondrial function.
Intracellular enzyme activity and dye retention is a hallmark of viable cells. During cell death, increased plasma membrane permeability causes enzyme leakage and a reduction in activity. Dyes that measure esterase activity are modified with ester-moieties, which help them passively diffuse through cell membranes and render the dyes non-fluorescent. Once inside the cell, ubiquitous esterases present in the cytosol cleave ester-moieties, activating dye fluorescence and trapping the dye within the cell. ION sells Calcein AM in multiple formats (dry or in a prepared solution) and in combination with Ethidium Homodimer I as part of ION Vital – Viability so you can simultaneously detect dead cells.
(Ex/Em 495 nm/515 nm) – Calcein AM is a membrane-permeant, non-fluorescent form of calcein that is converted to green fluorescent calcein in viable cells, resulting in uniform cytosolic fluorescence. Calcein is well retained within the cytosol of most healthy cells with intact cell membranes.
This kit combines Ethidium Homodimer I and Calcein AM to provide a two-color, fluorescence-based assay to discriminate between live and dead cells.
A loss of plasma membrane integrity is an indicator of cell death. Nucleic acid-binding, cell impermeant dyes, like Ethidium Homodimer I (EthD-I), can be used to label the nuclei of cells with compromised plasma membranes. These dyes are excluded from intact, healthy cells and exhibit minimal fluorescence in the absence of nucleic acids. Once the dye crosses the cell membrane and binds to nucleic acids (DNA) inside the cell, it displays a significant increase in fluorescence. ION sells EthD-I in multiple formats (dry or in a prepared solution) and in combination with Calcein AM as part of ION Vital – Viability so you can simultaneously detect viable cells.
(Ex/Em: 528 nm/617 nm) – EthD-I is a membrane-impermeable, high-affinity, nucleic acid stain that selectively labels dead cells with compromised plasma membranes.
This kit combines Ethidium Homodimer I and Calcein AM to provide a two-color, fluorescence-based assay to discriminate between live and dead cells.
(Ex/Em: 490,540 nm/525, 590nm) – JC-10 is a mitochondrial membrane potential probe. It possesses superior aqueous solubility compared to its better known analogue, JC-1. At low concentrations, JC-10 is monomeric and emits a green fluorescence. JC-10 accumulates in healthy mitochondria, forming J-aggregates that exhibit an orange fluorescence. Its potentiometric behavior allows for ratiometric analysis of mitochondrial membrane potential, where a shift from orange to green fluorescence is indicative of compromised mitochondria.
MitoVolt is a mitochondrial membrane potential assay kit that uses JC-10, a potentiometric dye that is more soluble than JC-1. It is an ideal solution for detecting changes in mitochondrial membrane potential due to cell apoptosis or other stress-inducing phenomena, and is compatible with fluorescence microscopy, flow cytometry, and plate reader applications.
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ION VITAL - Viability Kit
Two-color, fluorescence-based assay to discriminate between live and dead cells. |
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$242.00
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ION VITAL - MitoVolt
Mitochondrial membrane potential assay kit that uses JC-10, a dye that accumulates in polarized mitochondria where it emits a red-shifted fluorescence, that is more soluble than JC-1. 5 plate. |
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$308.00
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BCECF AM
Ratiometric, green fluorescent pH indicator, membrane permeable. |
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$108.00
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Ethidium Homodimer I
Red fluorescent, dead cell stain. |
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$165.00 – $198.00
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Calcein AM
Calcein AM is a membrane-permeant, non-fluorescent indicator that is converted to green fluorescent calcein by esterase activity in viable cells. |
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$109.00 – $165.00
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JC-10 in DMSO (5 x 50 µL)
JC-10 allows for ratiometric analysis of mitochondrial membrane potential, where a shift from orange (Ex/Em: 540nm/590nm) to green fluorescence (Ex/Em: 490nm/525nm) is indicative of compromised mitochondria.
5 x 50 μL. |
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$248.00
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