Store – ION Biosciences https://ionbiosciences.com Fluorescent Ion Indicators | Assay Kits Thu, 12 Nov 2026 20:31:14 +0000 en-US hourly 1 https://wordpress.org/?v=7.1.4 https://ionbiosciences.com/wp-content/uploads/ION-Bio-100x100.png Store – ION Biosciences https://ionbiosciences.com 32 32 OG488-HTL https://ionbiosciences.com/store/og488-htl/ https://ionbiosciences.com/store/og488-htl/#respond Thu, 20 Aug 2026 17:41:03 +0000 https://ionbiosciences.com/?post_type=product&p=36289 OG488-HTL is a photostable, green-fluorescent HaloTag® ligand for any intracellular protein expressing HaloTag® fusion protein. This ligand is extremely pH stable in the physiological range and down to mildly acidic conditions (pKa ~ 4.6), allowing direct covalent labeling. Suitable for localization studies and offering a highly stable complex, easily track the protein of interest and follow its cellular transport.

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OG488-HTL is a photostable, green-fluorescent HaloTag® ligand for any intracellular protein expressing HaloTag® fusion protein. This ligand is extremely pH stable in the physiological range and down to mildly acidic conditions (pKa ~ 4.6), allowing direct covalent labeling. Suitable for localization studies and offering a highly stable complex, easily track the protein of interest and follow its cellular transport.

Data

Chemigenetic pH sensors combine precise genetic targeting with interchangeable synthetic fluorophores, enabling selection of the optimal color, photostability, and pKₐ for each application.

OG488-HTL (in green) localized in lysosomes expressing HaloTag®, multiplexed with 6-TMR-HTL (red). Cells were treated with the TMEM175 activator DCPIB and imaged every 30 minutes for 5 hours on a Cytation 5
]]> https://ionbiosciences.com/store/og488-htl/feed/ 0 6-TMR-HTL https://ionbiosciences.com/store/6-tmr-htl/ https://ionbiosciences.com/store/6-tmr-htl/#respond Wed, 24 Jun 2026 16:25:38 +0000 https://ionbiosciences.com/?post_type=product&p=35533

6-TMR HaloTag® Ligand (6-TMR-HTL) is a cell-permeable fluorescent labeling reagent designed for the covalent labeling of HaloTag fusion proteins in live or fixed cells. The bright 6-tetramethylrhodamine (6-TMR, 6-TAMRA) fluorophore enables sensitive detection of HaloTag®-tagged proteins. Upon binding, the ligand forms a stable covalent bond with the HaloTag® enzyme, providing long-term signal retention and low background fluorescence. 6-tetramethylrhodamine is conjugated to the HaloTag® chloroalkane ligand through a PEG-2 linker.

6-TMR HaloTag® Ligand is ideal for live-cell imaging, protein localization studies, receptor internalization assays, protein trafficking analysis, HaloTag reporter assays, fluorescence microscopy, confocal imaging, flow cytometry, and high-content screening.

Data

HEK293T cells overexpressing TMEM175 stained with ION Biosciences’ 6-TMR HaloTag.
HEK293T cells transduced with Mito-HaloTag and stained with ION Biosciences’ 6-TMR-HTL.
]]> https://ionbiosciences.com/store/6-tmr-htl/feed/ 0 Brilliant Lithium Assay https://ionbiosciences.com/store/brilliant-lithium-assay/ https://ionbiosciences.com/store/brilliant-lithium-assay/#respond Wed, 11 Feb 2026 19:49:41 +0000 https://ionbiosciences.com/?post_type=product&p=35018 ION Biosciences' Brilliant Lithium Assay is a next-generation, fluorescence-based approach for high-throughput screening of NaV1.X channel modulators. The assay uses ION Lithium Green 1 AM (ILG-1 AM), a membrane-permeable, lithium-sensitive fluorescent dye that enables functional voltage-gated sodium channel measurements using lithium as a surrogate ion. Lithium readily permeates open NaV1.X channels while bypassing many endogenous sodium-handling mechanisms, resulting in a robust and selective readout of channel activity. Upon depolarization or pharmacological activation of NaV1.X channels, lithium accumulates intracellularly producing a rapid, quantifiable fluorescence increase that reflects real time channel activity.]]>

Voltage-gated sodium channels (NaV1.X) are essential membrane proteins that initiate and propagate action potentials by mediating rapid sodium influx in response to membrane depolarization. They underlie key physiological processes including neuronal signaling (NaV1.1, NaV1.2, NaV1.6), cardiac conduction (NaV1.5), and pain sensation (NaV1.7–NaV1.9). Abnormal NaV1.X activity is implicated in disorders such as epilepsy, cardiac arrhythmias, and chronic pain, positioning these channels as high-value therapeutic targets. Conventional methods for assessing NaV1.X function such as patch-clamp electrophysiology and radiolabeled ion flux assays are often low-throughput, technically demanding, and reliant on specialized instrumentation. 

ION Biosciences’ Brilliant Lithium Assay overcomes these limitations with a next-generation, fluorescence-based approach for high-throughput screening of NaV1.X channel modulators. The assay uses ION Lithium Green 1 AM (ILG-1 AM), a membrane-permeable, lithium-sensitive fluorescent dye that enables functional NaV1.X channel measurements using lithium as a surrogate ion. Lithium readily permeates open NaV1.X channels while bypassing many endogenous sodium-handling mechanisms, resulting in a robust and selective readout of channel activity. Upon depolarization or pharmacological activation of NaV1.X channels, lithium accumulates intracellularly producing a rapid, quantifiable fluorescence increase that reflects real time channel activity.

Compared to our Brilliant Sodium 2 Assay, Brilliant Lithium delivers approximately a two-fold larger screening window, enhanced assay sensitivity, and a larger dynamic range. This improved performance supports reliable modulator identification in both 96- and 384-well high-throughput screening formats.

Data

Sodium channels act as the primary transport pathway for lithium into cells. Our Lithium-selective sensor, ILG-1 AM, can measure changes in intracellular lithium concentration associated with the activity of voltage-dependent sodium channels.

Brilliant Lithium supports hit finding with large signal amplitudes and the largest screening window of any sodium channel assay. A Nav1.8 inhibition assay reveals kinetic traces with fluorescence fold changes approaching 5X and 3X larger screening windows than Brilliant Sodium 2. 

Brilliant Lithium can be used to identify pore blockers and state-dependent inhibitors of voltage-gated sodium channels using either a dual addition or single addition protocol. Here is a dose response curve of suzetrigine for Nav1.8.

]]> https://ionbiosciences.com/store/brilliant-lithium-assay/feed/ 0 ILG-1 AM https://ionbiosciences.com/store/ilg-1-am/ https://ionbiosciences.com/store/ilg-1-am/#respond Wed, 11 Feb 2026 19:47:45 +0000 https://ionbiosciences.com/?post_type=product&p=35020 ION Lithium Green 1 AM (ILG-1 AM), is a membrane-permeable, lithium-sensitive fluorescent indicator that enables solution- and cell-based lithium detection. ILG-1 is a high affinity and highly selective lithium sensor for fluorescence-based, lithium quantitation, with the largest fluorescence intensity enhancement upon binding to lithium. It can also be used for sodium channel drug discovery, as lithium readily permeates open NaV1.X channels resulting in a robust and selective readout of sodium channel activity.]]>

ION Lithium Green 1 AM (ILG-1 AM), is a membrane-permeable, lithium-sensitive fluorescent dye that enables solution- and cell-based lithium detection. Due to its high affinity and selectivity for lithium ions, ILG-1 enables rapid determination of lithium concentrations in a variety of samples compared to the other commercial lithium assays. 

As a key component in our Brilliant Lithium Assay, ILG-1 AM is also compatible with intracellular lithium detection and can be used for functional NaV1.X channel screening where lithium is a surrogate ion for sodium. Lithium readily permeates open NaV1.X channels while bypassing many endogenous sodium-handling mechanisms, resulting in a robust and selective readout of channel activity. Upon depolarization or pharmacological activation of NaV1.X channels, lithium accumulates intracellularly producing a rapid, quantifiable fluorescence increase that reflects real time channel activity.

Data

ILG-1 AM, in its active form, displays a robust fluorescence increase to lithium in solution (approximately 10-fold) with an approximate Kd of 422 µM.

ILG-1 AM, in its active form, displays high selectivity over other monovalent cations over comparable concentration ranges. 

ILG-1 AM, in its active form, displays high selectivity for lithium when compared to physiologically relevant monovalent and divalent cations. With respect to divalent cations, ILG-1 appears to bind manganese exclusively, albeit not as well as its preferred lithium ion. 

]]> https://ionbiosciences.com/store/ilg-1-am/feed/ 0 Brilliant Intracellular pH Assay https://ionbiosciences.com/store/brilliant-intracellular-ph-assay/ https://ionbiosciences.com/store/brilliant-intracellular-ph-assay/#respond Tue, 10 Feb 2026 17:58:01 +0000 https://ionbiosciences.com/?post_type=product&p=34972 ION Biosciences’ Brilliant Intracellular pH Assay is a high-throughput, no-wash, fluorescence-based solution optimized to monitor activation of plasma membrane proton-coupled channels and transporters in response to pharmacological compounds. These include the voltage-gated proton channel (HV1), Na⁺/H⁺ exchangers (SLC9), divalent metal transporters (SLC11), peptide transporters (SLC15), monocarboxylate transporters (SLC16), proton-coupled amino acid transporters (SLC36), proton-coupled sugar transporters (SLC45), and the proton-coupled folate transporter (SLC46). Kit contains enough reagents to conduct 10 plate-based assays.]]>

Intracellular pH (pHᵢ) is a fundamental regulator of numerous cellular processes, including cell growth, enzymatic activity, receptor-mediated signal transduction, ion transport, endocytosis, and cell adhesion. Dysregulated pHᵢ is associated with a variety of diseases, highlighting the need for reliable tools to measure dynamic pH changes in live cells.

ION Biosciences’ Brilliant Intracellular pH Assay is a high-throughput, no-wash, fluorescence-based platform optimized to monitor activation of plasma membrane proton-coupled channels and transporters in response to pharmacological compounds. These include the voltage-gated proton channel (HV1), Na+/H+ exchangers (SLC9), divalent metal

transporters (SLC11), peptide transporters (SLC15), monocarboxylate transporters (SLC16), proton-coupled amino acid transporters (SLC36), proton-coupled sugar transporters (SLC45), and the proton-coupled folate transporter (SLC46).

The assay uses the cell-permeable dye BCECF AM, a green fluorescent pH indicator with a pKa of ~7 and dual-excitation properties (Ex/Em ~430/535 nm and ~490/535 nm). The ratio of these emission intensities is directly proportional to the intracellular pH and can be used to quantify co-transport of other substrates in proton-coupled SLC transporters. Ratiometric measurement enables precise determination of absolute pHᵢ while minimizing variability from photobleaching, dye loading, or cell morphology. For high-throughput screening, BCECF can also be measured non-ratiometrically using standard fluorescein settings. The assay delivers sensitive, quantitative readouts with strong signal and broad dynamic range, compatible with 96- and 384-well plates, making it ideal for mechanistic studies, dose–response profiling, and large-scale screening campaigns.

Data

Measure the activity of proton-dependent SLC transporters like PepT1 in response to substrate addition in realtime, and quantify important compound effects such as substrate affinity.

Identify if compounds are substrates or modulators for specific SLC transporters or H+ channels, like TMEM175.

Use intracellular pH calibration solutions to correlate readouts to specific intracellular pH values.

]]> https://ionbiosciences.com/store/brilliant-intracellular-ph-assay/feed/ 0 HEK293T Kv1.3 (KCNA3) Cell Line https://ionbiosciences.com/store/hek293t-kv1-3-kcna3-cell-line/ https://ionbiosciences.com/store/hek293t-kv1-3-kcna3-cell-line/#respond Fri, 19 Dec 2025 16:03:10 +0000 https://ionbiosciences.com/?post_type=product&p=34930 A recombinant cell line that stably expresses human Kv1.3 (KCNA3) and provides a cell-based model to support discovery and profiling of Kv1.3 inhibitors.

Check out our validated assay formats for this target: Thallium-free GIRK Potassium Channel Assay and Brilliant Thallium Assay (fluorescence-based).

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Kv1.3 (KCNA3) is a voltage-gated potassium channel that plays a critical role in the immune system. It regulates T-cell activation by maintaining membrane potential, making it a key target for treating autoimmune diseases. It is also implicated in other conditions, such as cancer and metabolic disorders.

This product is a cell-based, fluorescence-compatible model with stable expression in HEK293T cells, supporting FLIPR, Panoptic and Flexstation workflows in the therapeutic areas noted for this target (Immunology, Cancer, CNS).

Data

Dalazatide dose-response curve for human Kv1.3 channel inhibition using a thallium-free potassium fluorescence assay in HEK293T cells.
Dalazatide dose-response curve for a Kv1.3 channel inhibition assay using
ION’s Thallium-free GIRK potassium channel assay.
]]> https://ionbiosciences.com/store/hek293t-kv1-3-kcna3-cell-line/feed/ 0 BAPTA JF™519-HTL https://ionbiosciences.com/store/bapta-jf519-htl/ Thu, 04 Dec 2025 20:51:59 +0000 https://ionbiosciences.com/?post_type=product&p=34869 BAPTA JFTM519-HTL is a yellow fluorescent (Ex/Em = 520/550 nm), intracellular calcium indicator that displays a chloroalkane ligand for binding to Halotag. The Halotag ligand enables localization to specific cell phenotypes in mixed cell populations and intracellular organelles using Halotag technology. Halotag expressing cells are required when using this dye.

*JF™ is a trademark owned by the Howard Hughes Medical Institute. Janelia Fluor® is a Registered Trademark of the Howard Hughes Medical Institute.

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BAPTA JFTM519-HTL is a yellow fluorescent (Ex/Em = 520/550 nm), intracellular calcium indicator that displays a chloroalkane ligand for binding to Halotag. The Halotag ligand enables localization to specific cell phenotypes in mixed cell populations and intracellular organelles using Halotag technology. Halotag expressing cells are required when using this dye.

*JF™ is a trademark owned by the Howard Hughes Medical Institute. Janelia Fluor® is a Registered Trademark of the Howard Hughes Medical Institute.

]]> HEK293 Kv11.1 (hERG) Cell Line https://ionbiosciences.com/store/hek293-kv11-1-herg-cell-line/ https://ionbiosciences.com/store/hek293-kv11-1-herg-cell-line/#respond Mon, 25 Aug 2025 19:39:29 +0000 https://ionbiosciences.com/?post_type=product&p=34412 Check out our Thallium-free hERG Potassium Channel Assay for a highly-sensitive, robust, and high-throughput method to measure hERG potassium channel activity.

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ION Biosciences’ HEK293 Kv11.1 (hERG) recombinant cell line stably expresses human KCNH2 and provides a robust, well-validated model for investigating Kv11.1 channel function. This cell line is ideally suited for high-throughput screening of channel modulators, safety pharmacology assays, and mechanistic studies aimed at discovering novel inhibitors or activators of Kv11.1. With excellent reproducibility and functional expression, these cells facilitate efficient evaluation of compound effects on Kv11.1 currents using fluorescence-based assays.

Voltage-gated potassium (Kv) channels are integral membrane proteins that enable selective potassium ion permeation in response to changes in cellular membrane potential. These channels play critical roles in shaping action potentials, regulating cellular excitability, and maintaining ion homeostasis across diverse tissues. The human genome encodes 40 Kv channels, categorized into 12 subfamilies (Kv1–Kv12), each exhibiting unique biophysical properties and tissue distribution.


Kv11.1, also known as hERG (human Ether-à-go-go Related Gene), is a key member of the Kv channel family encoded by the KCNH2 gene. This channel is predominantly expressed in cardiac myocytes and the central nervous system, where it is essential for repolarizing the cardiac action potential and regulating neuronal excitability. Dysfunction or pharmacological inhibition of Kv11.1 channels leads to delayed cardiac repolarization, manifesting as drug-induced long QT syndrome—a potentially fatal arrhythmia of major concern in drug development. Because of this, regulatory agencies including the FDA mandate routine screening of new drug candidates for Kv11.1 inhibition. Beyond cardiac physiology, Kv11.1 channels have emerging roles in neurological disorders, tumor proliferation, and cellular migration, making them a valuable target for a broad range of therapeutic applications.

Data

The biological function of the HEK293 Kv11.1 (hERG) recombinant cell line was validated using terfenadine, a well-characterized tool compound known to inhibit hERG function. Functional activity of hERG channels was measured using ION Biosciences’ Brilliant Thallium Gold Assay, Brilliant Thallium Gold Snapshot Assay, and Thallium-Free hERG Potassium Channel Assay kits. These kits use fluorescent ion indicators to detect the flow of thallium or potassium ions through the channel. HEK293 Kv11.1 (hERG) recombinant cells were pre-incubated with the fluorescent ion indicators’ optimized loading conditions, followed by exposure to increasing concentrations of terfenadine. A dose-dependent change in fluorescence was observed upon addition of a membrane-depolarizing stimulus solution, reflecting inhibition of Kv11.1-mediated ion flux. Fluorescence was recorded continuously following compound addition, and the resulting data were analyzed to determine IC50 values based on maximum velocity (Vmax) or area under the curve (AUC). The assay produced an IC50 consistent with literature values for terfenadine, confirming functional expression and pharmacological responsiveness of Kv11.1 channels in the HEK293 cell line.

hERG assay (Kv11.1) using thallium flux to identify inhibitors displays a dose-dependent response to terfenadine, a potent hERG inhibitor. Kinetic traces and a concentration response curve are shown.
hERG assay (Kv11.1) using thallium-free potassium channel assay to identify inhibitors displays a dose-dependent response to terfenadine, a potent hERG inhibitor. Kinetic traces and a concentration response curve are shown.
hERG channel assay (Kv11.1) using thallium snapshot to identify inhibitors displays a dose-dependent response to terfenadine, a potent hERG inhibitor. Kinetic traces and a concentration response curve are shown.
]]> https://ionbiosciences.com/store/hek293-kv11-1-herg-cell-line/feed/ 0 HEK293T GAT1 (SLC6A1) Cell Line https://ionbiosciences.com/store/hek293t-gat1-slc6a1-cell-line/ https://ionbiosciences.com/store/hek293t-gat1-slc6a1-cell-line/#respond Thu, 07 Aug 2025 19:04:51 +0000 https://ionbiosciences.com/?post_type=product&p=34290 HEK293T GAT1 (SLC6A1) recombinant cell line stably expresses the human GABA transporter and provides a validated in vitro model for studying GAT1 function and pharmacology. This cell line is optimized for high-throughput screening of GAT1 inhibitors or substrates, functional uptake assays, and kinetic studies of transporter activity. Its strong, reproducible sodium-dependent GABA uptake and compatibility with fluorescence-based assays make it an ideal platform for advancing drug discovery efforts targeting GABAergic pathways in CNS disease. Check out our Sodium-dependent SLC Transporter Assay for a highly-sensitive, robust, and high-throughput method to measure sodium-dependent SLC transporter activity.]]>

ION Biosciences’ HEK293T GAT1 (SLC6A1) recombinant cell line stably expresses the human GABA transporter and provides a validated in vitro model for studying GAT1 function and pharmacology. This cell line is optimized for high-throughput screening of GAT1 inhibitors or substrates, functional uptake assays, and kinetic studies of transporter activity. Its strong, reproducible sodium-dependent GABA uptake and compatibility with fluorescence-based assays make it an ideal platform for advancing drug discovery efforts targeting GABAergic pathways in CNS disease.

The GABA transporter 1 (GAT1), encoded by the SLC6A1 gene, is a sodium- and chloride-dependent symporter responsible for the high-affinity reuptake of γ-aminobutyric acid (GABA) from the synaptic cleft into presynaptic neurons and surrounding glial cells. By regulating extracellular GABA concentrations, GAT1 plays a key role in maintaining inhibitory tone and synaptic homeostasis throughout the central nervous system, particularly in regions such as the hippocampus, cortex, and cerebellum. Dysregulation of GAT1 function has been implicated in a variety of neurological and psychiatric disorders, including epilepsy, autism spectrum disorder, anxiety, and schizophrenia.

SLC6A1 is a member of the SLC6 family of neurotransmitter transporters and shares the conserved 12-transmembrane domain topology characteristic of this family. GAT1 couples the transport of GABA with the inward movement of sodium and chloride ions, leveraging electrochemical gradients to drive uptake against concentration gradients. This mechanism is essential for the rapid clearance of GABA from synapses and for shaping inhibitory signaling dynamics.

graphs showing uptake of GABA by GAT1 with SLC transporter assay
]]> https://ionbiosciences.com/store/hek293t-gat1-slc6a1-cell-line/feed/ 0 Thallium-free hERG Potassium Channel Assay https://ionbiosciences.com/store/thallium-free-herg-potassium-channel-assay/ https://ionbiosciences.com/store/thallium-free-herg-potassium-channel-assay/#respond Fri, 01 Aug 2025 14:56:12 +0000 https://ionbiosciences.com/?post_type=product&p=34260 IPG-1 AM, our lowest affinity potassium indicator to measure changes in intracellular potassium concentration. This kit is optimized for hERG screening (Kv11.1), and can be used to identify activators and inhibitors of hERG channels.]]>

To facilitate safer and physiologically relevant evaluation of hERG channel activity, ION Biosciences has developed a Thallium-Free hERG Potassium Channel Assay utilizing ION Potassium Green 1 AM (IPG-1 AM), a membrane-permeable, fluorescent dye sensitive to intracellular potassium levels. 

Upon membrane depolarization, hERG channels open and mediate potassium efflux, causing a decrease in intracellular potassium concentration. This drop in intracellular potassium levels is detected in real time as a reduction in IPG-1 fluorescence intensity, providing a direct measure of channel function.

This direct-readout eliminates the need for surrogate ions or radioactive tracers and enables robust, high-throughput screening to identify hERG modulators in 96- and 384-well formats. 

Series of graphics showing how the Thallium-free hERG Channel Assay works

The Thallium-Free hERG Potassium Channel Assay provides reliable EC50/IC50 values and is ideally suited for pharmacological profiling, cardiotoxicity safety screening, and therapeutic discovery for hERG channels. 

This kit has not yet been validated for use with additional voltage-gated potassium channels at this time. 

All data displayed was generated using our stable HEK293 hERG cell line. 

Identify inhibitors: Compounds that inhibit hERG activity, such as terfenadine, do not display a loss of fluorescence after treatment with a depolarizing stimulus solution to activate the channel.

Kinetic traces and corresponding concentration response curve showing hERG inhibition by terfenadine measured using the thallium-free hERG channel assay.

Large screening windows in inhibitor modes with Z’ > 0.9 to enable reliable inhibitor detection. 

Graphs showing kinetic traces of hERG expressing cells treated with or without terfenadine, and corresponding Z' calculation in a 384-well format.

Titrate stimulus solution for optimal screening windows: Adding different concentrations of  the depolarizing stimulus solution can result in more channel activity and greater potassium efflux.

Graphs showing kinetic traces of IPG-1 fluorescence in hERG expressing cells after treatment with various concentrations of a depolarizing stimulus solution.
]]> https://ionbiosciences.com/store/thallium-free-herg-potassium-channel-assay/feed/ 0 ION Thallos-HTL https://ionbiosciences.com/store/ion-thallos-htl/ https://ionbiosciences.com/store/ion-thallos-htl/#respond Fri, 11 Jul 2025 20:54:19 +0000 https://ionbiosciences.com/?post_type=product&p=34228 A localizable thallium indicator that can be selectively targeted to individual cells or organelles using HaloTag® technology. ION Thallos-HTL is a ligand for the HaloTag® enzyme. It can be used to conduct cell-specific potassium channel assays in mixed cell populations, or to probe organellar ion channels in their native environment. Cells expressing HaloTag® are required when using ION Thallos-HTL.]]>
A localizable thallium indicator that can be selectively targeted to individual cells or organelles using HaloTag® technology. ION Thallos-HTL is a ligand for the HaloTag® enzyme. It can be used to conduct cell-specific potassium channel assays in mixed cell populations, or to probe organellar ion channels in their native environment. Cells expressing HaloTag® are required when using ION Thallos-HTL.
Sample images of cell-specific thallium flux assays using Halotag expression and ION Thallos-HTL
Sample images of nucleus targeted thallium dye using Halotag expression and ION Thallos-HTL
Sample assay of cell-selective thallium flux measurements on a high throughput screening instrument using ION Thallos-HTL
]]> https://ionbiosciences.com/store/ion-thallos-htl/feed/ 0 Brilliant Sodium 2 Assay Kit https://ionbiosciences.com/store/brilliant-sodium-2-assay/ https://ionbiosciences.com/store/brilliant-sodium-2-assay/#respond Tue, 17 Jun 2025 22:26:49 +0000 https://ionbiosciences.com/?post_type=product&p=34085

Brilliant Sodium 2 Assay is a second-generation, fluorescence-based solution designed for high-throughput screening of voltage-gated sodium channel (NaV1.X) modulators. Activation of NaV1.X channels by membrane depolarization or direct stimulation causes a rapid influx of sodium ions, leading to a robust fluorescence increase. This change provides a sensitive and quantitative readout of NaV1.X channel activity and pharmacological modulation. 

Just like the original Brilliant Sodium Assay, this version directly measures sodium influx using ING-2 AM, a sodium-sensitive fluorescent indicator. Compared to the original version, Brilliant Sodium 2 has been reengineered to deliver:

  • Larger signal amplitudes
  • Expanded screening windows for more robust assay performance


This product is well-suited for determining EC50 or IC50 values of NaV1.X activators and inhibitors in 96- and 384-well formats for high throughput screening of voltage-gated sodium channels.

Data

Use dual addition protocols to identify inhibitors: Compounds that inhibit NaV1.3 and NaV1.5 activity, such as tetracaine, can be identified by following a dual-addition protocol.

Nav inhibitor screening results generated using Brilliant Sodium Assay 2. Kinetic traces and tetracaine dose response curves are displayed.
Expanded screening windows in activator and inhibitor modes compared to Brilliant Sodium in NaV1.3 and NaV1.5 assays.
]]> https://ionbiosciences.com/store/brilliant-sodium-2-assay/feed/ 0 Thallium-free GIRK Potassium Channel Assay https://ionbiosciences.com/store/thallium-free-girk-potassium-channel-assay/ Tue, 03 Jun 2025 21:09:38 +0000 https://ionbiosciences.com/?post_type=product&p=34007 Thallium-free GIRK Potassium Channel Assay is the first fluorescence-based potassium channel solution that directly measures potassium flux, enabling you to move away from thallium-containing products. This product features the potassium-sensitive dye, IPG-1 AM, our lowest affinity potassium indicator to measure changes in intracellular potassium concentration. This kit is optimized for GIRK (Kir3.x) screening, and can be used to identify activators and inhibitors of GIRK channels.]]>

Traditional approaches for measuring GIRK activity such as patch clamp, FRET, or thallium flux assays can be labor-intensive, low-throughput, or reliant on toxic surrogate ions. To overcome these limitations, ION Biosciences built the Thallium-Free GIRK Potassium Channel Assay featuring ION Potassium Green-1 AM (IPG-1 AM), a membrane-permeable, potassium-sensitive, fluorescent indicator that directly measures potassium flux.

Upon binding of an agonist to Gi/o-coupled GPCRs or direct activation of GIRK channels, potassium ions flow through the channel and out of the cell, resulting in a measurable decrease in intracellular potassium concentration. This change in potassium concentration is detected in real-time by IPG-1 as a loss of fluorescence intensity. This direct-readout eliminates the need for surrogate ions or radioactive tracers and enables robust, high-throughput screening to identify GIRK modulators in 96- and 384-well formats. 

The Thallium-Free GIRK Potassium Channel Assay provides reliable EC50/IC50 values and is ideally suited for pharmacological profiling and discovery of therapeutic compounds targeting GIRK channels or GIRK channel-coupled pathways.

This kit has not been validated for use with additional potassium channel targets at this time.

GIRK potassium channel assay illustration

Data

All data displayed was generated using our stable HEK293 – huKir3.1/3.2 cell line. 

Identify activators: Compounds that increase GIRK activity, such as ML297, exhibit a concentration dependent loss of intracellular fluorescence, indicative of K+ efflux.

GIRK potassium channel assay to identify Kir3 inhibitors. Kinetic traces and dose response curves for SCH23390, a selective GIRK inhibitor.

Identify inhibitors: Compounds that inhibit GIRK activity, such as SCH23390, do not display a loss of fluorescence upon treatment with a potent activator such as ML297.

Large screening windows in activator and inhibitor modes with Z’ > 0.8 enable reliable hit-finding campaigns.

GIRK potassium channel assay displays high Z’ values for hit identification in activator and inhibitor screening modes
]]> HEK293T GlyT1 (SLC6A9) Recombinant Cell Line https://ionbiosciences.com/store/hek293t-glyt1-slc6a9-recombinant-cell-line/ Wed, 16 Apr 2025 20:32:47 +0000 https://ionbiosciences.com/?post_type=product&p=33779 HEK293T GlyT1 (SLC6A9) Recombinant Cell Line  is a stable, recombinant HEK293T cell line expressing GlyT1 (SLC6A9), validated using glycine uptake assays and suitable for identifying modulators of human GlyT1 activity. Be sure to check out our new Sodium-dependent SLC Transporter Assay for screening SLCs that co-transport sodium.]]>

Sodium-dependent glycine transporter 1 (GlyT1, encoded by SLC6A9) is a transmembrane transporter responsible for the reuptake of glycine, the major inhibitory neurotransmitter in the central nervous system. GlyT1 is a member of the SLC6 family of sodium- and chloride-dependent neurotransmitter transporters and operates by coupling glycine transport with the co-transport of sodium and chloride ions across the plasma membrane. The transporter plays a key role in regulating extracellular glycine concentrations, particularly in glycinergic synapses and in modulating N-methyl-D-aspartate (NMDA) receptor activity through glycine clearance.

The human genome encodes two primary glycine transporters—GlyT1 (SLC6A9) and GlyT2 (SLC6A5)—with distinct expression patterns and physiological functions. Human GlyT1 is predominantly expressed in glial cells throughout the brain and spinal cord and is essential for maintaining glycine homeostasis in excitatory synapses. Dysregulation of GlyT1 function has been implicated in various neurological and psychiatric disorders, including schizophrenia, epilepsy, and cognitive dysfunction. As such, GlyT1 is a promising pharmacological target for the development of novel therapeutics aimed at modulating glycinergic and glutamatergic neurotransmission

ION Biosciences’ HEK293T GlyT1 (SLC6A9) recombinant cell line stably expresses the human glycine transporter 1 and is functionally validated using glycine uptake assays. This model is ideally suited for identifying and profiling small molecule modulators of hGlyT1 activity in both high throughput screening and detailed pharmacological studies.

Fluorescence data showing inhibition of GlyT1 with ALX 5407
Glycine uptake kinetic traces in GlyT1 expressing HEK293T cells
]]> Sodium-Dependent SLC Transporter Assay https://ionbiosciences.com/store/sodium-dependent-slc-transporter-assay/ https://ionbiosciences.com/store/sodium-dependent-slc-transporter-assay/#respond Wed, 26 Mar 2025 19:01:20 +0000 https://ionbiosciences.com/?post_type=product&p=33629 ION’s Sodium-dependent SLC Transporter Assay detects SLC transporter activity of SLCs that co-transport sodium, such as the SLC6 transporter family. This assay is ideal for hit finding and lead optimization applications in drug discovery, and can be used to discover substrates, potentiators, and inhibitors of sodium-dependent SLC transporters. Included buffers are not compatible with many sugar transporters, including SGLTs. If you'd like to evaluate sugar transporters Contact Us and we can provide custom buffer formulations for your target.]]>

ION Biosciences’ Sodium-dependent SLC Transporter Assay utilizes ION Natrium Green 2 (ING-2 AM), a sodium-sensitive fluorescent indicator, to detect solute carrier (SLC) transporter mediated sodium influx. This assay kit provides a highly-sensitive, robust, and high-throughput method to measure SLC transporter activity with benefits including:

  • Measures sodium, the native ion ensuring on target activity.

  • Can be used to identify substrates, potentiators and inhibitors of sodium-dependent SLC transporters.

  • Generates comparable EC50 and IC50 values to electrophysiology.

  • Compatible with electrogenic and electroneutral SLC transporters that cotransport sodium.


Optimized for high-throughput screening in 96- and 384-well formats, ION’s Sodium-dependent SLC Transporter Assay kit ensures reliability and reproducibility, making it ideal for hit finding and lead optimization applications. Backed by ION’s exceptional technical support, this kit simplifies your research workflow by providing ready-to-use buffers and simple protocols designed by the experts you’ve learned to trust.

Illustration of active sodium-depdendent SLC transporter showing sodium (Na⁺) flux and solute movement across the cell membrane, measured using ING-2 AM.

Data

Measure substrate uptake in real-time as demonstrated in kinetic traces of various GABA concentrations to GAT1 (SLC6A1) expressing cells.

Illustration of active sodium-depdendent SLC transporter showing sodium (Na⁺) flux and solute movement across the cell membrane, measured using ING-2 AM.

Validated uptake assays for multiple Sodium-dependent SLC transporters, including SLC6A1 and SLC6A9, among other targets.

GABA uptake assay in GAT1 (SLC6A1) expressing cells at varying GABA concentrations using ION’s Sodium-dependent SLC Transporter Assay
Glycine uptake assay in GLyT1 (SLC6A9) expressing cells at varying glycine concentrations using ION’s Sodium-dependent SLC Transporter Assay

The Sodium-dependent SLC assay can also be used to identify inhibitors of transporter function, as showcased in a 384-well assay for SLC6A9 (GlyT1), with IC50s comparable to reported literature values.

Real-time kinetic traces showing ALX 5407 activity in SLC6A9 (GlyT1) expressing cells across multiple concentrations
Glycine transporter inhibitor screen data showing a dose-response curve of ALX 5407 in SLC6A9 transporter cells.
High-throughput GABA transporter inhibition assay (SLC6A1) using NNC-711. Z-factor of 0.78 demonstrates assay robustness for SLC6A1 inhibitor hit identification.

Achieve exceptional Z’ whether you use AUC or Vmax analysis, ensuring this kit is ready for your next SLC hit-finding campaign.

]]> https://ionbiosciences.com/store/sodium-dependent-slc-transporter-assay/feed/ 0 ION Thallos Gold-HTL https://ionbiosciences.com/store/ion-thallos-gold-htl/ https://ionbiosciences.com/store/ion-thallos-gold-htl/#respond Tue, 26 Nov 2024 21:28:45 +0000 https://ionbiosciences.com/?post_type=product&p=33082 A localizable thallium indicator that can be selectively targeted to individual cells or organelles using HaloTag® technology. ION Thallos Gold-HTL is a ligand for the HaloTag® enzyme, and displays red-shifted excitation and emission relative to ION Thallos-HTL. It can be used to conduct cell-specific potassium channel assays in mixed cell populations, or to probe organellar ion channels in their native environment. Cells expressing HaloTag® are required when using this product. ]]>

Thallos Gold is a gold fluorescent, intracellular thallium (Tl+) indicator. Thallium indicators have been the gold standard for fluorescence-based potassium (K+) channel HTS for nearly 2 decades. ION Thallos Gold-HTL offers the opportunity to localize the indicator to individual cells within a mixed cell population or intracellular compartments using HaloTag® technology, without compromising its Tl+ sensitivity. Once ION Thallos Gold-HTL is loaded into cells expressing HaloTag® protein, the indicator covalently binds to the protein. Addition of stimulus solution will show ion flux within the cell type or intracellular compartment of interest.

Cells expressing HaloTag® are required when using this product.

High throughput screening assay of ION Thallos Gold-HTL treated cells displays only a signal increase in Halotag positive cells
Nuclear localization of ION Thallos Gold-HTL for organellar ion channel assays
luorescence images of ION Thallos Gold-HTL loaded cells. Cells expressing halotag are significantly brighter than wild-type cells.
]]> https://ionbiosciences.com/store/ion-thallos-gold-htl/feed/ 0 BAPTA JF™549-HTL https://ionbiosciences.com/store/bapta-jf-549-htl/ Thu, 24 Oct 2024 17:15:42 +0000 https://ionbiosciences.com/?post_type=product&p=33023 BAPTA JF™549-HTL is a calcium indicator that also displays the Halotag ligand for cell-specific and organellar localization using Halotag technology. A Janelia Fluor® dye backbone provides excellent brightness and photostability, making it useful for measuring fast calcium dynamics in neurons and cardiomyocytes. This calcium indicator also outperforms red-shifted genetically encoded calcium sensors in sensitivity and fluorescence intensity.]]>

BAPTA JF549-HTL is a red fluorescent (Ex/Em = 546/569 nm), intracellular calcium indicator that displays a chloroalkane ligand for binding to Halotag. The Halotag ligand enables localization to specific cell phenotypes in mixed cell populations and intracellular organelles using Halotag technology. Designed with a Janelia Fluor® dye backbone, the calcium indicator displays excellent brightness and photostability for measuring fast calcium dynamics in neurons and cardiomyocytes. It also outperforms red-shifted genetically encoded calcium sensors in sensitivity and fluorescence intensity. The red-shifted fluorescence minimizes tissue autofluorescence, enables multicolor imaging, and permits the use of optogenetic tools for triggering calcium transients. Halotag expressing cells are required when using this dye.

*JF™ is a trademark owned by the Howard Hughes Medical Institute. Janelia Fluor® is a Registered Trademark of the Howard Hughes Medical Institute.

Fluorescence images of BAPTA JF549-HTL loaded cells. Cells expressing halotag are significantly brighter than wild-type cells.
]]> CHO K1 Kv11.1 (hERG) Recombinant Cell Line https://ionbiosciences.com/store/chok1-kv11-1-herg-cell-line/ https://ionbiosciences.com/store/chok1-kv11-1-herg-cell-line/#respond Wed, 17 Jul 2024 14:19:06 +0000 https://ionbiosciences.com/?post_type=product&p=32850  A stable CHO cell line overexpressing hERG potassium channels for studying Kv11.1 pharmacology and safety screening. Be sure to check out our Brilliant Thallium Assay and or Thallium-free hERG Potassium Channel Assay for high throughput formatted kits, suitable for voltage-gated potassium channels    ]]>

Our CHO-K1 Kv11.1 (hERG) Recombinant Cell Line is a high-performance, stably expressing cell for cardiotoxicity screening and hERG drug discovery. Validated using fluorescence-based assays, like Brilliant Thallium Assay, and Automated Patch Clamp (APC) platforms, including the SyncroPatch 384, this essential cell line provides complete coverage for your hERG screening needs.

hERG channels (Kv11.1, KCNH2) are essential for cardiac repolarization, and their inhibition is a primary concern in drug safety assessment due to the risk of long QT syndrome and potential fatal arrhythmias. Given the FDA’s stringent CiPA (Comprehensive in Vitro Proarrhythmia Assay) guidelines, rigorous hERG screening is a critical step in drug development to identify off-target cardiac effects.

Key Features & Applications

  • Validated for Automated Patch Clamp – Compatible with high-throughput electrophysiology platforms like SyncroPatch 384
  • High-Quality Ion Channel Expression – Reliable Kv11.1 (hERG) expression with industry-standard pharmacological response
  • Ideal for Cardiac Safety & Drug Discovery – Supports electrophysiology-based assays, fluorescence-based screening (e.g., thallium flux assays), and CiPA-compliant testing
  • Broad Applications Beyond Cardiac Screening – hERG channels are also implicated in schizophrenia, tumor proliferation, cell migration, and apoptosis, expanding their relevance beyond cardiotoxicity studies

Optimized for Industry-Standard Drug Screening

Using this cell line in high-throughput electrophysiology and fluorescence assays allows for precise IC₅₀ determinations of known hERG inhibitors, ensuring alignment with regulatory expectations and literature benchmarks. Recent pharmacological validation with Bepridil, Diltiazem, Ranolazine, and Verapamil confirmed its reliability in predicting hERG-related drug interactions across multiple assay formats and temperature conditions.

Ensure your cardiac safety and ion channel screening workflows are backed by the highest-quality recombinant hERG cell line.

Representative hERG assay data for high-throughput screening of Kv11.1 modulators, such as terfenadine.

Our hERG-expressing CHO K1 cells were also validated with automated electrophysiology using the SyncroPatch 384 from Nanion Technologies, highlighting ION’s CHO K1 hERG cell line is an excellent tool for cardiac safety profiling.

  • High success rates (96%) for cells with R Seal >500MΩ.
  • Results agree with reported literature values.
  • Patch stable for >10 min, and compatible with room and physiological temperature recordings.
Read the full application note here
]]> https://ionbiosciences.com/store/chok1-kv11-1-herg-cell-line/feed/ 0 BAPTA JF™549 https://ionbiosciences.com/store/bapta-jf-549/ Wed, 17 Jul 2024 02:29:14 +0000 https://ionbiosciences.com/?post_type=product&p=32851

BAPTA JF549 is a red fluorescent (Ex/Em = 546/569 nm), intracellular calcium indicator. Designed with a Janelia Fluor® dye backbone, it displays excellent brightness and photostability for measuring fast calcium dynamics in neurons and cardiomyocytes. It also outperforms red-shifted genetically encoded calcium sensors in sensitivity and overall fluorescence intensity. The red-shifted fluorescence minimizes tissue autofluorescence and also enables multicolor imaging and the use of optogenetic tools for triggering calcium transients.

*JF™ is a trademark owned by the Howard Hughes Medical Institute. Janelia Fluor® is a Registered Trademark of the Howard Hughes Medical Institute.

Imaging action potentials in neurons via calcium imaging with BAPTA JF549.
Data showing improved brightness and photostability of Janelia Fluor dyes compared to TMR.
Measuring optogenetically driven calcium transients in iPSC-derived cardiomyocytes
]]> HEK293 – huKir3.1/3.2 Cell Line https://ionbiosciences.com/store/hek293-hukir3-1-3-2-cell-line/ Thu, 22 Feb 2024 15:32:00 +0000 https://ionbiosciences.com/?post_type=product&p=32501 A stable HEK cell line overexpressing GIRK potassium channels for studying Kir3.1/3.2 pharmacology and Gi/o GPCRs. Be sure to check out our Gi/o GPCR-GIRK Thallium Flux Assay or our new Thallium-free GIRK Potassium Channel Assay to discover modulators of GIRK potassium channels ]]> A stable HEK cell line overexpressing GIRK potassium channels for studying Kir3.1/3.2 pharmacology and Gi/o GPCRs. 

]]> Gi/o GPCR-GIRK Thallium Flux Assay https://ionbiosciences.com/store/gi-o-gpcr-girk-thallium-flux-assay/ Wed, 31 Jan 2024 22:38:00 +0000 i/o GPCR assay for discovering modulators through direct coupling to GIRK channels. Cell lines and thallium flux reagents are included.]]>

ION’s Gi/o GPCR-GIRK Thallium Flux Assay is a total assay solution for multi-well plate-based, high-throughput measurements of Gi/o GPCR agonism measured via native GIRK coupling. This kit provides most reagents needed, including a stable HEK293 cell line that expresses human Kir3.1 and Kir3.2, to conduct Gi/o GPCR HTS. A simple transduction step allows the flexibility to study a plethora of GPCRs that couple to GIRK using thallium flux.

Key Benefits:

  • Native coupling
  • Does not require artificial stimulation
  • Flexibility to insert any Gi/o GPCR
  • Discover additional hits
  • Uncover biased agonists
  • Explore complex pharmacology

GPCR-GIRK Thallium Flux Assay Principle.

Image of a cell membrane displaying a GIRK channel, or inward rectifying potassium channel
Image of a cell membrane displaying a Gi GPCR and GIRK channel, coupled through the alpha subunit
Image of a cell membrane displaying a Gi GPCR and GIRK channel, coupled through the alpha subunit
Gi GPCR GIRK Thallium flux assay principle, showing GPCR ligand binding activated GIRK channels, which enables thallium to go into the cell and increases fluorescence of Thallos

Data

Dose-response curves for four Gi GPCR, g-protein coupled receptors, (Dopamine D2, 5HT1A, M2, and Delta opioid) with EC50 values labeled, showing activation vs. ligand concentration.

Study any Gi/o GPCR

Insert any Gi/o GPCR of interests using transient or stable expression systems for rapid hit finding. Generate HTS results for your target within 24 hours. 

  • Rapid results
  • Ultimate flexibility
Two dose-response plots showing dopamine effects on dopamine D2 receptors. The left graph shows cAMP response with an EC50 of 5.5 µM. The right graph displays GPCR-GIRK assay results with an EC50 of 6.7 nM. Both graphs plot response intensity against log dopamine concentration.

cAMP versus Gi/o GPCR-GIRK Assay

As demonstrated in this dopamine (D2) receptor example, cAMP assays can be complicated by the combination of Gs and Gi GPCR receptor expression. HEK cells endogenously express multiple dopamine receptors, Our Gi/o GPCR-GIRK Thallium Flux Assay readout is specific to Gi GPCR signaling, enabling you to ignore the noise and hone in on your target of interest.

  • No artificial stimulation needed
  • Measure Gi receptor signalling only

cAMP versus Gi/o GPCR-GIRK Assay

Unlock better assay quality with Z’ out-matching cAMP assays. When studying Gi-coupled receptors which inhibit cAMP production, the high basal cAMP levels induced by forskolin can make it difficult to detect small decreases in cAMP caused by receptor activation.

  • Higher Z’
  • Larger signal amplitude
Gi GPCR assay data for Dopamine D2 receptor, including a kinetic trace and dose response curve
Gi GPCR high throughput screening data for Dopamine D2 receptor, including a kinetic trace and Z’ analysis for hit discovery.
Gi GPCR 96-well dose response curves for Dopamine D2 receptor, Serotonin receptor (5HT1A), muscarinic receptor (M2), and delta opioid receptor.
Gi GPCR 384-well dose response curves for Serotonin receptor (5HT1A), muscarinic receptor (M2), and delta opioid receptor.

Learn more

New to thallium flux assays, read our blog called How to analyze thallium flux assay data.

Interested in learning how ION’s Gi/o GPCR-GIRK Thallium Flux Assay can help you discover a brighter future?

Talk to us with us today for more information or to request a sample.
Book a meeting
]]> Brilliant Thallium https://ionbiosciences.com/store/brilliant-thallium-assay/ Thu, 02 Nov 2023 21:20:00 +0000 https://ionbiosciences.com/store/brilliant-thallium/

ION Biosciences’ Brilliant Thallium (Tl⁺) Assay is a total assay solution for multi-well plate-based, high-throughput measurements of Tl⁺ flux through K⁺, Na⁺, non-selective cation channels, and some Na⁺ or K⁺ transporters. The ION Biosciences’ Brilliant Thallium Assay is also useful for a wide range of effectors of ion channels and transporters including G protein-coupled receptors, lipid and protein kinase, for example. 

In multi-well, plate-based formats, the Brilliant Thallium Assay can be used to discover and characterize the effects of tens to many tens-of-thousands of compounds and environmental factors on effectors of Tl⁺ flux. Over the past 15 years, fluorescence-based measures of Tl⁺ flux have been widely utilized to discover small molecule modulators of a host of ion channels, transporters, GPCRS and other targets of interest for both drug discovery and basic research.

Our Flex kit provides all the necessary reagents in a format that maximizes flexibility for your assay design and optimization needs.

Our Express kit is custom tailored to customer’s needs, providing only the reagents you need in the amounts you need them. Less waste, higher reproducibility, and more convenience.

Data

How ION’s Brilliant Thallium Assay Outperforms the Competition

ION Biosciences Brilliant Thallium Assay excels in potassium channel assays, standing out among market leaders for its robust signal amplitude, high Z’, customizability, and cost-effectiveness.

Potassium channel assay dose response curve comparing Thallos AM to Fluxor made by ThermoFisher
Thallos AM outperforms ThermoFisher´s FluxOR dye in potassium channel assay
  • Higher Signal: 50% larger response
  • Better Z´: Clearly distinguish active from inactive compounds
  • Trusted by Industry: Validated by Large Pharmaceutical Company
Brilliant Thallium has a larger signal amplitude than FluxOR II Green
  • Greater sensitivity: Larger response means you can detect activity with confidence
  • Cost-Effective:Use less thallium per well, saving you more money for HTS campaigns
Potassium channel assay comparing Brilliant Thallium to Fluxor II Green made by ThermoFisher
Molecular Devices’ FLIPR Potassium Channel Assay compared to ION’s Brilliant Thallium Assay presented as a concentration response curve
FLIPR Potassium Channel assay (left) vs. Brilliant Thallium (right)
  • Assay Quality: Comparable Z´values ensure reliable hit identification
  • Comparable Potencies: IC50 values match Molecular Devices´kit across multiple compounds
  • Flexibility: Brilliant Thallium offers flexibility to run wash and no wash formats in the same box

Examples of ion channel and transporter assays

ION Biosciences’ Brilliant Thallium Assay is more than just a potassium channel solution. It can also be used to uncover modulators of voltage-gated sodium channels, transporters like KCC, non-selective cation channels, and Gi/o GPCRs.

Fluorescence profile of voltage gated sodium channel (Nav1.3) activation using veratridine with corresponding concentration response curve. Data acquired using a FLIPR-compatible Brilliant Thallium Assay kit
Fluorescence profile of voltage gated sodium channel (Nav1.3) inhibition using tetracaine with corresponding concentration response curve. Data acquired using a FLIPR-compatible Brilliant Thallium Assay kit
Brilliant thallium flux assay used for voltage-gated potassium channels. Data show fluorescence kinetic profiles and corresponding dose response curve of hERG channel inhibition.
Brilliant thallium flux assay used for voltage-gated potassium channels. Data show fluorescence kinetic profiles and corresponding dose response curve of KCNQ inhibition.
Brilliant thallium flux assay used for inward-rectifying potassium channels. Data show fluorescence kinetic profiles and corresponding dose response curve of GIRK activation.

Experience Superior Performance

ION’s Brilliant Thallium Assay isn’t just another option—it’s a superior solution for potassium channel assays and more. With Thallos AM at its core, our kit outperforms industry leaders like ThermoFisher and has key advantages against Molecular Devices too. Offering enhanced signal amplitude, greater sensitivity, flexibility, and cost-effectiveness, Brilliant Thallium is designed to deliver reliable, high-quality data for your research.

Learn more

First time using thallium flux assays to measure ion channel activity? Read our step-by-step guide for how to turn raw kinetic traces into informative dose response curves.

Have a GFP-expressing ion channel, check out Brilliant Thallium Gold, our Brilliant Thallium Assay packaged with Thallos Gold, a red-shifted thallium indicator for projects demanding something other than a green dye.

Interested in learning how ION’s Brilliant Thallium Assay can help you discover a brighter future?

Talk to us with us today for more information or to request a sample.
Book a meeting
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