SQT1 – ION Biosciences https://ionbiosciences.com Fluorescent Ion Indicators | Assay Kits Wed, 29 Oct 2025 16:39:40 +0000 en-US hourly 1 https://wordpress.org/?v=7.0.4 https://ionbiosciences.com/wp-content/uploads/ION-Bio-100x100.png SQT1 – ION Biosciences https://ionbiosciences.com 32 32 CHO K1 Kv11.1 (hERG) Recombinant Cell Line https://ionbiosciences.com/store/chok1-kv11-1-herg-cell-line/ https://ionbiosciences.com/store/chok1-kv11-1-herg-cell-line/#respond Wed, 17 Jul 2024 14:19:06 +0000 https://ionbiosciences.com/?post_type=product&p=32850  A stable CHO cell line overexpressing hERG potassium channels for studying Kv11.1 pharmacology and safety screening. Be sure to check out our Brilliant Thallium Assay and or Thallium-free hERG Potassium Channel Assay for high throughput formatted kits, suitable for voltage-gated potassium channels    ]]>

Our CHO-K1 Kv11.1 (hERG) Recombinant Cell Line is a high-performance, stably expressing cell for cardiotoxicity screening and hERG drug discovery. Validated using fluorescence-based assays, like Brilliant Thallium Assay, and Automated Patch Clamp (APC) platforms, including the SyncroPatch 384, this essential cell line provides complete coverage for your hERG screening needs.

hERG channels (Kv11.1, KCNH2) are essential for cardiac repolarization, and their inhibition is a primary concern in drug safety assessment due to the risk of long QT syndrome and potential fatal arrhythmias. Given the FDA’s stringent CiPA (Comprehensive in Vitro Proarrhythmia Assay) guidelines, rigorous hERG screening is a critical step in drug development to identify off-target cardiac effects.

Key Features & Applications

  • Validated for Automated Patch Clamp – Compatible with high-throughput electrophysiology platforms like SyncroPatch 384
  • High-Quality Ion Channel Expression – Reliable Kv11.1 (hERG) expression with industry-standard pharmacological response
  • Ideal for Cardiac Safety & Drug Discovery – Supports electrophysiology-based assays, fluorescence-based screening (e.g., thallium flux assays), and CiPA-compliant testing
  • Broad Applications Beyond Cardiac Screening – hERG channels are also implicated in schizophrenia, tumor proliferation, cell migration, and apoptosis, expanding their relevance beyond cardiotoxicity studies

Optimized for Industry-Standard Drug Screening

Using this cell line in high-throughput electrophysiology and fluorescence assays allows for precise IC₅₀ determinations of known hERG inhibitors, ensuring alignment with regulatory expectations and literature benchmarks. Recent pharmacological validation with Bepridil, Diltiazem, Ranolazine, and Verapamil confirmed its reliability in predicting hERG-related drug interactions across multiple assay formats and temperature conditions.

Ensure your cardiac safety and ion channel screening workflows are backed by the highest-quality recombinant hERG cell line.

Representative hERG assay data for high-throughput screening of Kv11.1 modulators, such as terfenadine.

Our hERG-expressing CHO K1 cells were also validated with automated electrophysiology using the SyncroPatch 384 from Nanion Technologies, highlighting ION’s CHO K1 hERG cell line is an excellent tool for cardiac safety profiling.

  • High success rates (96%) for cells with R Seal >500MΩ.
  • Results agree with reported literature values.
  • Patch stable for >10 min, and compatible with room and physiological temperature recordings.
Read the full application note here
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