ing – ION Biosciences https://ionbiosciences.com Fluorescent Ion Indicators | Assay Kits Fri, 16 Jan 2026 19:29:46 +0000 en-US hourly 1 https://wordpress.org/?v=7.1.3 https://ionbiosciences.com/wp-content/uploads/ION-Bio-100x100.png ing – ION Biosciences https://ionbiosciences.com 32 32 ING-1 AM https://ionbiosciences.com/store/ing-1-am/ Wed, 28 Jun 2023 19:27:05 +0000 https://ionbiosciences.com/?post_type=product&p=26726

ION Natrium Green – 1 (ING-1) AM is a membrane permeable, yellow-green fluorescent, intracellular sodium (Na) indicator. Formerly known as Asante Natrium Green – 1 (ANG-1). ING-1 AM has improved cellular loading and significantly higher brightness than SBFI. Compatible with GFC and YFP filters.

ING-1 (ION natrium green-1) banner describing that it is a low affinity sodium indicator

excitation and emission spectra of ING-1 (ION natrium green-1), a sodium indicator
Fluorescence of ING-1 (ion natrium green-1), a sodium indicator, increases with sodium concentration.
Concentration response curve of ouabain, an inhibitor of the sodium/potassium-pump, shows increasing ING-1 fluorescence at higher concentrations
ING-1_Monovalent_Divalent-v2
]]> ING-2 AM https://ionbiosciences.com/store/ing-2-am/ Wed, 28 Jun 2023 19:26:48 +0000 https://ionbiosciences.com/?post_type=product&p=26645

ING-2 AM (Ion natrium green - 2), a fluorescent sodium indicator, general features banner

ION Natrium Green – 2 (ING-2) is a yellow-green fluorescent, intracellular sodium (Na⁺) indicator with improved cellular loading and significantly higher brightness than SBFI. Its spectral properties (Ex/Em: 525 nm/545 nm) and large dynamic range make ING-2 the best Na⁺ indicator for high-throughput screening applications targeting Na⁺ channels, and non-selective monovalent cation channels. ING-2 has a more physiologically relevant affinity (Kd = 20 mM) than ING-1 (Kd = 92 mM). Also compatible with fluorescence microscopy using common fluorescein, GFP or more ideally YFP filters.

 
Fluorescence emission data of ING-2 binding to sodium ion (Na+) at different concentrations, and a sodium binding curve.
Dose response curve and fluorescence images of ouabain treated cells loaded with ING-2. Ouabain inhibits the sodium potassium pump (Na/K-ATPase).
Ouabain concentration response curve acquired using ING-2 AM, showing inhibition of the sodium-potassium pump (Na/K-ATPase).
Plotted change in fluorescence of ING-2 in response to sodium concentration
Measuring sodium channel activity using the sodium indicator, ING-2 AM. Inactivation of Nav1.3, a voltage gated sodium channel, is blocked by veratridine, resulting in an increase in cellular fluorescence.
ING-2 Response to Divalent Ions.
]]> ING-2 TMA⁺ Salt https://ionbiosciences.com/store/ing-2-tma-salt/ Wed, 28 Jun 2023 19:26:48 +0000 https://ionbiosciences.com/?post_type=product&p=26646

ION Natrium Green – 2 (ING-2) is a yellow-green fluorescent, intracellular sodium (Na⁺) indicator with improved cellular loading and significantly higher brightness than SBFI. Its spectral properties (Ex/Em: 525 nm/545 nm) and large dynamic range make ING-2 the best Na⁺ indicator for high-throughput screening applications targeting Na⁺ channels, and non-selective monovalent cation channels. ING-2 has a more physiologically relevant affinity (Kd = 20 mM) than ING-1 (Kd = 92 mM). Also compatible with fluorescence microscopy using common fluorescein, GFP or more ideally YFP filters.

ING-2 TMA⁺ salt is a membrane impermeable form of ING-2 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.

ING-2 salt, a fluorescent sodium indicator, banner
ING-2 Response to Divalent Ions.
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