fluo – ION Biosciences https://ionbiosciences.com Fluorescent Ion Indicators | Assay Kits Fri, 25 Jul 2025 16:47:19 +0000 en-US hourly 1 https://wordpress.org/?v=7.1.4 https://ionbiosciences.com/wp-content/uploads/ION-Bio-100x100.png fluo – ION Biosciences https://ionbiosciences.com 32 32 Fluo-Gold AM https://ionbiosciences.com/store/fluo-gold-am/ Wed, 28 Jun 2023 19:27:00 +0000 https://ionbiosciences.com/?post_type=product&p=26704

Fluo-Gold is a gold fluorescent (Ex/Em = 528/548 nm), intracellular calcium indicator. Designed to measure intracellular Ca²⁺ flux in high throughput screening and fluorescence microscopy applications. Fluo-Gold’s red-shifted excitation and emission enable multiplexing with GFP-expressing cells or other green fluorescent indicators and minimizes interference from green-fluorescent compounds.

A red-shifted calcium indicator as shown in excitation and emission spectra and representative fluorescence images in a calcium mobilization assay
Fluo-gold, a gold fluorescent calcium indicator, outperforms fluo-4 when using GFP-expressing cells. Overlaid fluorescence images of GFP and fluo-gold are shown.
]]> Fluo-3 AM https://ionbiosciences.com/store/fluo-3-am/ Wed, 28 Jun 2023 19:26:33 +0000 https://ionbiosciences.com/?post_type=product&p=26586 Fluo-3 is a green fluorescent, intracellular calcium (Ca²⁺) indicator with slightly red-shifted spectral properties compared to Fluo-4. Offers a large dynamic range that can be better suited for high contrast imaging applications. Is preferred over Fluo-4 for some cell types.

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]]> Fluo-3 K⁺ Salt https://ionbiosciences.com/store/fluo-3-k-salt/ Wed, 28 Jun 2023 19:26:32 +0000 https://ionbiosciences.com/?post_type=product&p=26583 Fluo-3 is a green fluorescent, intracellular calcium (Ca²⁺) indicator with slightly red-shifted spectral properties compared to Fluo-4. Offers a large dynamic range that can be better suited for high contrast imaging applications. Is preferred over Fluo-4 for some cell types.

Fluo-3 K⁺ salt is a membrane impermeable form of Fluo-3 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.

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]]> Fluo-4 K⁺ Salt https://ionbiosciences.com/store/fluo-4-k-salt/ Wed, 28 Jun 2023 19:26:32 +0000 https://ionbiosciences.com/?post_type=product&p=26584 Fluo-4 is the most popular green fluorescent, calcium (Ca²⁺) indicator. Proven and trusted for decades to measure intracellular Ca²⁺ flux in high throughput screening and fluorescence microscopy applications.

Fluo-4 K⁺ Salt is a membrane impermeable form of Fluo-4 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.

Fluo-4 salt, a calcium indicator, banner highlighting it's widespread use

]]> Fluo-2 AM https://ionbiosciences.com/store/fluo-2-am/ Wed, 28 Jun 2023 19:26:32 +0000 https://ionbiosciences.com/?post_type=product&p=26580 Fluo-2 is a green fluorescent, intracellular calcium (Ca²⁺) indicator. Fluo-2 has higher Ca²⁺ affinity (Kd = 290 nM), superior cell loading and comparable spectral properties compared to Fluo-4. Offers superior sensitivity versus other Fluo analogues at lower Ca²⁺ concentrations.

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