SPECIFICATIONS
Excitation:
340 
380 nm 
Emission:
505 nm 
Kd:
4 mM
MW:
1127
Solubility:
DMSO
Synonyms: Sodium-binding benzofuran isophthalate – AM, sodium binding indicator
Product code: 1mg: 2021B / 20x 50mg: 2021E
product description
SBFI is a UV-excitable, ratiometric green indicator for intracellular sodium (Na⁺) measurements. Ex/Em: 340/505 nm can be used to measure Na⁺-bound SBFI, and Ex/Em: 380/505 nm can be used to detect Na⁺-free SBFI. It is ~18X more selective for Na⁺ over K⁺. Ratiometry is optimal for imaging applications where quantification of intracellular Na⁺ concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.
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