SPECIFICATIONS
Excitation:
340 
380 nm 
Emission:
505 nm 
Kd:
5 mM
Synonyms: Potassium-binding benzofuran isophthalate – AM, Potassium binding indicator
Product code: 1mg: 3033B / 20x 50mg: 3033E
product description
PBFI is a UV-excitable, ratiometric green indicator for intracellular potassium (K⁺) measurements. Ex/Em: 340/505 nm can be used to measure K⁺-bound PBFI, and Ex/Em: 380/505 nm can be used to detect K⁺-free PBFI. It is 1.5X more selective for K⁺ over Na⁺and works well in cytosolic conditions where [K⁺]/[Na⁺] is ~10. Ratiometry is optimal for imaging applications where quantification of intracellular K⁺ concentrations is desired and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.
PBFI K⁺ salt is a membrane impermeable form of PBFI that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection, or other methods.
documentation
