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Fura-2 K+ Salt Product Specifications

Cell-permeable acetoxymethyl (AM) ester derivative of Fura-2, a UV-excitable, excitation-ratiometric calcium indicator.

Molecular Weight

832g/mol

CAS#

132319-57-4

Solubility

H2O

Kd

145 nM

Odor

None

Fire and Explosion Hazards

None

Toxicology Data

Not known

Handling and Storage

Store at -20°C. Protect from light and moisture

Shelf Life

Valid for one year after delivery, if stored properly

TLC on Reverse Phase Plate

Reverse phase C18 on silica

Solvent

4:6 Water/Methanol

Rf

Solvent front

HPLC

Column

C18

Detector Settings

254 nm, 360 nm

Purity

> 90%

Absorbance Spectrum

Solvent

100 mM KCl, 10 mM MOPS, pH 7.2 (low calcium)

Absorbance max

363 ± 3 nm

ε

28000 M-1cm-1

Fluorescence Spectrum

Solvent

100 mM Kcl, 10 mM MOPS, pH 7.2 (low calcium)

Excitation max

363 nm

Emission max

505 nm ± 3 nm

1H NMR

All relevant peaks present

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Fura-2 K+ Salt Results

Fura-2 is one of the first commercial fluorescent calcium indicators introduced by Dr. Tsien and produced by Molecular Probes in 1986. It is now so common in the field that it appears in standard molecular cell biology textbooks.

UV-excitable Calcium Indicators

Catalog Number

MW (g/mol)

Absorbance (nm)

Excitation (nm)

Emission (nm)

Kda (nm)

Solubility

Fura-2 AM

1051B 1051E

1002 340/380 nm UV

371

N/A*

N/A*

N/A*

DMSO

Fura-2 K+ Salt

1052B 1052E

832

354

340/380
(high/low CA
2+)

505

145

H20

*Fura-2 K+ Salt values are obtained after hydrolysis by non-specific esterases in the cell

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