Fura-2 AM Documentation
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Fura-2 AM Product Specifications
Cell-permeable acetoxymethyl (AM) ester derivative of Fura-2, a UV-excitable, excitation-ratiometric calcium indicator.
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Molecular Weight |
1002 g/mol |
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Solubility |
DMSO |
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Kd |
145 nM |
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† |
Fluorescence changes due to calcium binding are observed only after hydrolysis of AM esters by non-specific esterases to the Fura-2 pentanion. |
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Odor |
None |
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Fire and Explosion Hazards |
None |
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Toxicology Data |
Not known |
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Handling and Storage |
Store at -20°C. Protect from light and moisture |
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Shelf Life |
Valid for one year after delivery, if stored properly |
TLC |
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Solvent |
2:1 Ethyl Acetate/Hexanes |
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Rf |
0.5 |
HPLC |
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Column |
C18 |
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Detector Settings |
254 nm, 371 nm |
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Purity |
> 95% |
Absorbance Spectrum |
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Solvent |
Ethyl Acetate |
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Absorbance max |
371 ± 3 nm |
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ε |
33000 M-1cm-1 |
Fluorescence Spectrum |
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Solvent |
Ethyl Acetate |
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Excitation max |
371 nm ± 3 nm |
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Emission max |
471 nm ± 3 nm |
Mass Spectrum |
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Electrospray Ionization |
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Mass-to-Charge Ratio |
1002 M/Z |
1H NMR |
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All relevant peaks present |
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Solvent |
Deuterated acetone |
Fura-2 AM Results
Fura-2 is one of the first commercial fluorescent calcium indicators introduced by Dr. Tsien and produced by Molecular Probes in 1986. It is now so common in the field that it appears in standard molecular cell biology textbooks.
UV-excitable Calcium Indicators
| Catalog Number | MW (g/mol) | Absorbance (nm) | Excitation (nm) | Emission (nm) | Kda (nm) | Solubility | |
| Fura-2 AM | 1051B 1051E | 1002 340/380 nm UV | 371 | N/A* | N/A* | N/A* | DMSO |
| Fura-2 K+ Salt | 1052B 1052E | 832 | 354 | 340/380 (high/low CA2+) | 505 | 145 | H20 |
Fura-2 AM SDS & Info Packet
