Fluo-3 AM Documentation
Fluo-3 AM Product Specifications
Fluo-3 AM is a cell-permeable ester commonly used in cell-based experiments to detect changes in intracellular calcium levels.
|
Molecular Weight |
1129 g/mol |
|
Kd |
390 nM |
|
Odor |
None |
|
Fire and Explosion Hazards |
None |
|
Toxicology Data |
Not known |
|
Handling and Storage |
Store at -20°C. Protect from light and moisture |
|
Shelf Life |
Valid for six months after delivery, if stored properly |
TLC |
|
|
Solvent |
Ethyl Acetate / Hexane 2:1 |
|
Rf |
0.4 |
HPLC |
|
|
Column |
C18 |
|
Detector Settings |
254 nm, 371 nm |
|
Purity |
> 95% |
Absorbance Spectrum |
|
|
Solvent |
Ethyl Acetate |
|
Absorbance max |
460 nm |
Fluorescence Spectrum |
|
|
Fluoresces after hydrolyzed by esterases in the cell. |
|
|
Excitation max |
506 nm |
|
Emission max |
525 nm |
1H NMR |
|
|
All relevant peaks present |
|
|
Solvent |
Deuterated chloroform |
Fluo-3 AM Background
Most useful and inexpensive lasers operate in the green part of the visible spectrum and that has made green-emitting fluorescent calcium indicators very useful for confocal microscopy, flow cytometry, and screening. The popular green-emitting fluorescent calcium indicators for screening are Fluo-2, Fluo-3, Fluo-4, and Fluo-8. They are all derived from the original Fluo compound invented by Minta and Tsien (1989).
Fluo-3 has been used to image the spatial dynamics of Ca2+ signaling, in flow cytometry experiments involving photoactivation of caged chelators, second messengers, and neurotransmitters, and for cell-based pharmacological screening. Fluo-4 is an analog of Fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels. Cells may be loaded with the AM ester forms of these calcium indicators by adding the dissolved indicator directly to dishes containing cultured cells.
The patent for Fluo-2, Fluo-3, and Fluo-4 have expired so Ion Indicators offers these at reasonable prices.
Reagents for viability and Toxicity Assays
|
MW (g/mol) |
Excitation (nm) |
Emission (nm) |
Kd |
Extinction |
Solubilty |
|
|
Fluo-2 AM |
1061 |
N/A |
N/A |
N/A |
26,000 |
DMSO |
|
Fluo-2 K+ Salt |
891 |
490 |
494 |
230 |
80,000 |
H2O |
|
Fluo-3 AM |
1130 |
N/A |
N/A |
N/A |
26,000 |
DMSO |
|
Fluo-3 K+ Salt |
960 |
506 |
515 |
390 |
80,000 |
H2O |
|
Fluo-4 AM |
1097 |
N/A |
N/A |
N/A |
26,000 |
DMSO |
|
Fluo-4 K+ Salt |
927 |
490 |
526 |
345 |
80,000 |
H2O |
Fluo-3 AM SDS & Info Packet
