Fluo-2 High Affinity K+ Salt Documentation

Buy Fluo-2 High Affinity K+ Salt

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Fluo-2 K+ Salt High Affinity Product Specifications

Fluo-2 HighAff K+ Salt is a high affinity, green fluorescent Ca2+ binding dye.

Molecular Weight

891 g/mol

Kd

230 nM

Solubility

H2O

Odor

None

Fire and Explosion Hazards

None

Toxicology Data

Not known

Handling and Storage

Store at -20°C. Protect from light and moisture

Shelf Life

Valid for one year after delivery, if stored properly

TLC on Reverse Phase Plate

Solvent

H20 / Methanol 3:7

Rf

0.8

HPLC

Column

C18

Detector Settings

254 nm, 371 nm

Purity

> 95%

Fluorescence Spectrum

Excitation max

490 nm

Emission max

516 nm

1H NMR

All relevant peaks present

Solvent

Deuterated H2O

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Fluo-2 High Affinity K+ Salt Background

Fluo-2_HighAff_K+_Salt_Background

Most useful and inexpensive lasers operate in the green part of the visible spectrum and that has made green-emitting fluorescent calcium indicators very useful for confocal microscopy, flow cytometry, and screening. The popular green-emitting fluorescent calcium indicators for screening are Fluo-2, Fluo-3, Fluo-4, and Fluo-8. They are all derived from the original Fluo compound invented by Minta and Tsien (1989).

Fluo-2 is one of the original Fluo series of Ca2+ indicators invented by Roger Tsien in the 1980s. Whereas Fluo-3 and Fluo-4 were commercialized, Fluo-2 was not. Subsequently, Fluo-2 has been found to be much brighter (1.5x) than Fluo-4 in cellular experiments. The increased brightness of Fluo-2 is partly attributable to its superior loading in most cell types.

The patent for Fluo-2, Fluo-3, and Fluo-4 have expired so Ion Indicators offers these at reasonable prices.

Dye Characteristic Comparison

Reagents for viability and Toxicity Assays

MW (g/mol)

Excitation (nm)

Emission (nm)

Kd

Extinction

Solubilty

Fluo-2 AM

1061

N/A

N/A

N/A

26,000

DMSO

Fluo-2 K+ Salt

891

490

494

230

80,000

H2O

Fluo-3 AM

1130

N/A

N/A

N/A

26,000

DMSO

Fluo-3 K+ Salt

960

506

515

390

80,000

H2O

Fluo-4 AM

1097

N/A

N/A

N/A

26,000

DMSO

Fluo-4 K+ Salt

927

490

526

345

80,000

H2O

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