Fluo-2 High Affinity K+ Salt Documentation
Fluo-2 K+ Salt High Affinity Product Specifications
Fluo-2 HighAff K+ Salt is a high affinity, green fluorescent Ca2+ binding dye.
|
Molecular Weight |
891 g/mol |
|
Kd |
230 nM |
|
Solubility |
H2O |
|
Odor |
None |
|
Fire and Explosion Hazards |
None |
|
Toxicology Data |
Not known |
|
Handling and Storage |
Store at -20°C. Protect from light and moisture |
|
Shelf Life |
Valid for one year after delivery, if stored properly |
TLC on Reverse Phase Plate |
|
|
Solvent |
H20 / Methanol 3:7 |
|
Rf |
0.8 |
HPLC |
|
|
Column |
C18 |
|
Detector Settings |
254 nm, 371 nm |
|
Purity |
> 95% |
Fluorescence Spectrum |
|
|
Excitation max |
490 nm |
|
Emission max |
516 nm |
1H NMR |
|
|
All relevant peaks present |
|
|
Solvent |
Deuterated H2O |
Fluo-2 High Affinity K+ Salt Background
Most useful and inexpensive lasers operate in the green part of the visible spectrum and that has made green-emitting fluorescent calcium indicators very useful for confocal microscopy, flow cytometry, and screening. The popular green-emitting fluorescent calcium indicators for screening are Fluo-2, Fluo-3, Fluo-4, and Fluo-8. They are all derived from the original Fluo compound invented by Minta and Tsien (1989).
Fluo-2 is one of the original Fluo series of Ca2+ indicators invented by Roger Tsien in the 1980s. Whereas Fluo-3 and Fluo-4 were commercialized, Fluo-2 was not. Subsequently, Fluo-2 has been found to be much brighter (1.5x) than Fluo-4 in cellular experiments. The increased brightness of Fluo-2 is partly attributable to its superior loading in most cell types.
The patent for Fluo-2, Fluo-3, and Fluo-4 have expired so Ion Indicators offers these at reasonable prices.
Reagents for viability and Toxicity Assays
|
MW (g/mol) |
Excitation (nm) |
Emission (nm) |
Kd |
Extinction |
Solubilty |
|
|
Fluo-2 AM |
1061 |
N/A |
N/A |
N/A |
26,000 |
DMSO |
|
Fluo-2 K+ Salt |
891 |
490 |
494 |
230 |
80,000 |
H2O |
|
Fluo-3 AM |
1130 |
N/A |
N/A |
N/A |
26,000 |
DMSO |
|
Fluo-3 K+ Salt |
960 |
506 |
515 |
390 |
80,000 |
H2O |
|
Fluo-4 AM |
1097 |
N/A |
N/A |
N/A |
26,000 |
DMSO |
|
Fluo-4 K+ Salt |
927 |
490 |
526 |
345 |
80,000 |
H2O |
Fluo-2 K+ Salt High Affinity SDS & Info Packet
