Calcein AM Documentation

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Calcein AM Product Specifications

Fluorescent indicator in the cell permeable ester form.

CAS#

Solubility

Molecular Weight

995 g/mol

148504-34-1

DMSO

Handling and Storage

Store at -20°C. Protect from light and moisture

Shelf Life

Valid for one year after delivery, if stored properly

TLC

60Ǻ silica, 200 μm thickness

Solvent

Ethyl Acetate / Hexane 2:1

Rf

0.5

HPLC

Column

C18

Detector Settings

254 nm, 283 nm

Purity

> 95%

Absorbance Spectrum

Solvent

0.1N KOH in methanol

Absorbance max

503 ± 3 nm

ε

76,000 M-1cm-1

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Calcein AM Comparison

Calcein_Comparison

Dye Characteristic Comparison

Reagents for viability and Toxicity Assays

Catalog Number

MW (g/mol)

ABSmax

EXmax

Emmax

Solubility

BCECF (AM)*

0061

821

507

N/Ad

N/Ad

DMSO

Calcein (AM)

0066

995

494

N/Ad

N/Ad

DMSO

Calcein FREE ACID*

0067

623

494

494

517

H20

5(6) FAM Diacetate*

0305

460

492

N/Ad

N/Ad

DMSO

5(6) Carboxy DCFDA*

0503

531

504

N/Ad

N/Ad

DMSO

*Products only available via special request.

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