Assay Kits

Calcium Assay Kits

Over the last 30 years, Ca²⁺-sensitive indicators have found numerous uses ranging from high-spatial resolution imaging of synaptic activity to high-throughput GPCR assays.ION Biosciences’ Calcium Assay kits are a total assay solution for multi-well plate-based, high-throughput measurements of dynamic changes in intracellular Ca²⁺. Each assay kit is designed to be a simple, fast, and reliable fluorescence-based assay.

Whether your platform is a FLIPR, FlexStation®, or FDSS, ION’s assay kits will provide a smooth process for your assay development.

There are no intermediate wash-steps required with these kits. In each version, you will find all the reagents necessary for preparation to use as no-wash or washed assays for adherent and non-adherent cells.

Brilliant Thallium Assay Kits

ION Biosciences Brilliant Thallium Assays offer complete assay solutions for monovalent ion channel screening. Designed to measure Tl⁺ flux through potassium (K⁺), sodium (Na⁺), non-selective cation channels, and some Na⁺ or K⁺ transporters in plate-based, high-throughput formats.

Brilliant Thallium Assays are also useful to measure the activity of a wide range of effectors of ion channels and transporters, including G protein-coupled receptors (GPCR assays using thallium flux). In multi-well, plate-based formats, Brilliant Thallium Assays can be used to discover and characterize the effects of tens to many tens-of-thousands of compounds and environmental factors on potassium channels, sodium channels, and more. Thallium indicators are also available.

Brilliant Sodium Assay Kits

The only commercially available high-throughput, FLIPR sodium assay for measuring changes in intracellular Na⁺ mediated through a wide-variety of sodium channels and transporters. Designed as an intracellular, no wash, sodium flux assay with peak excitation at 525 nm and peak emission of 545 nm.

ION’s Brilliant Sodium Assay is compatible with fluorescence microscopes, flow cytometers, and plate readers capable of detecting FITC, GFP, or more optimally, yellow fluorescent protein (YFP). Sodium indicators are also available.

ION VITAL Assay Kits

ION Vital kits are designed to help you easily capture important markers of cell health, whether your preferred instrument is a plate reader, fluorescence microscope, or flow cytometer, using a collection of criteria that are indicative metrics of cell viability or death. These criteria include intracellular esterase activity as a marker of cell viability, plasma membrane integrity as a marker of cell death, and mitochondrial membrane potential as an early-stage marker of apoptosis. When used in combination, these assays can provide an informative snapshot of cell health.

Multidrug Resistance Activity Kit

ION’s Multidrug Resistance (MDR) Activity kit is an effective solution for detecting MDR1 and MRP1 activity and compounds susceptible to MDR-mediated efflux. ION’s MDR activity kit is compatible with fluorescence microscopy, flow cytometry and fluorescence microscopy, flow cytometry, and fluorescence plate readers using FITC/GFP settings.